Velocity measurement in microchannels with a laser confocal microscope and particle linear image velocimetry

Shih-Hui Chao, Mark R. Holl, John H. Koschwanez, Rob H. Carlson, Ling Sheng Jang, Deirdre Meldrum

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Particle linear image velocimetry (PLIV), a novel velocity measurement method for microfluidic characterization, is reported. The method records a series of one-dimensional images that represent the trace of particles in the flow across a one-dimensional imager. Linear imaging results in a faster frame rate than planar imaging, allowing observations at larger magnifications, or the measurement of faster flow in real-time than comparable techniques. In contrast to particle image velocimetry (PIV), PLIV captures fast flow dynamics and does not require high-speed cameras or shutters. Furthermore, PLIV is adaptable to multiple linear imager formats and, as one example, can use laser scanning confocal microscopes (LSCMs) that acquire images slowly, but with high optical sectioning resolutions. A higher spatial resolution can be obtained for flows where the in-plane velocity gradient in the direction of the optical path (z direction) is important. The PLIV algorithm is fully described and its utility is demonstrated by the measurement of: a steady Poiseuille flow with 1-μm spatial resolution in a microfluidic environment; dynamic measurement of transient flows with 250-ms temporal resolution; and the simultaneous calculation of particle dimension statistics.

Original languageEnglish (US)
Pages (from-to)155-160
Number of pages6
JournalMicrofluidics and Nanofluidics
Volume1
Issue number2
DOIs
StatePublished - May 2005
Externally publishedYes

Fingerprint

microchannels
Microchannels
velocity measurement
Velocity measurement
Microscopes
microscopes
Lasers
lasers
Microfluidics
Image sensors
Camera shutters
spatial resolution
Imaging techniques
high speed cameras
shutters
High speed cameras
Steady flow
particle image velocimetry
optical paths
magnification

Keywords

  • In situ measurement
  • Laser scanning confocal microscopy
  • Microfluidics
  • Particle linear image velocimetry

ASJC Scopus subject areas

  • Materials Science (miscellaneous)
  • Fluid Flow and Transfer Processes

Cite this

Velocity measurement in microchannels with a laser confocal microscope and particle linear image velocimetry. / Chao, Shih-Hui; Holl, Mark R.; Koschwanez, John H.; Carlson, Rob H.; Jang, Ling Sheng; Meldrum, Deirdre.

In: Microfluidics and Nanofluidics, Vol. 1, No. 2, 05.2005, p. 155-160.

Research output: Contribution to journalArticle

Chao, Shih-Hui ; Holl, Mark R. ; Koschwanez, John H. ; Carlson, Rob H. ; Jang, Ling Sheng ; Meldrum, Deirdre. / Velocity measurement in microchannels with a laser confocal microscope and particle linear image velocimetry. In: Microfluidics and Nanofluidics. 2005 ; Vol. 1, No. 2. pp. 155-160.
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