@article{f646e0c17e5e4c3e8d85937cebcc855f,
title = "Variable spontaneous mutation and loss of heterozygosity among heterozygous genomes in yeast",
abstract = "Mutation and recombination are the primary sources of genetic variation. To better understand the evolution of genetic variation, it is crucial to comprehensively investigate the processes involving mutation accumulation and recombination. In this study, we performed mutation accumulation experiments on four heterozygous diploid yeast species in the Saccharomycodaceae family to determine spontaneous mutation rates, mutation spectra, and losses of heterozygosity (LOH). We observed substantial variation in mutation rates and mutation spectra. We also observed high LOH rates (1.65-11.07×10-6 events per heterozygous site per cell division). Biases in spontaneous mutation and LOH together with selection ultimately shape the variable genome-wide nucleotide landscape in yeast species. ",
keywords = "Base substitutions, Diploid genome, GC content, LOH, Mutation rate, Mutational spectrum",
author = "Nguyen, {Duong T.} and Baojun Wu and Hongan Long and Nan Zhang and Caitlyn Patterson and Stephen Simpson and Krystalynne Morris and Thomas, {W. Kelley} and Michael Lynch and Weilong Hao",
note = "Funding Information: We would like to thank the associate editor and three anonymous reviewers for very helpful comments on a previous version of this article. We are grateful for the technical support from IEMB-1 computation cluster at OUC. This work was supported by grants from Pilot National Laboratory for Marine Science and Technology (Qingdao), the Marine S & T Fund of Shandong Province (No. 2018SDKJ0406-5) and Distinguished Scholars Support Program of Laboratory for Marine Biology and Biotechnology (YJ2019NO04), the Taishan Scholars Program for Early Career Experts of Shandong Province (tsqn201812024) to H.L., the Multidisciplinary University Research Initiative Award from the US Army Research Office (W911NF-09-1-0444) to M.L., P. Foster, H. Tang, and S. Finkel, and National Institutes of Health award (R35-GM122566) to M.L., and University Research Grant (from Wayne State University) to W.H. Publisher Copyright: {\textcopyright} 2020 The Author(s) 2020.",
year = "2020",
month = nov,
day = "1",
doi = "10.1093/molbev/msaa150",
language = "English (US)",
volume = "37",
pages = "3118--3130",
journal = "Molecular Biology and Evolution",
issn = "0737-4038",
publisher = "Oxford University Press",
number = "11",
}