Abstract
Mutation and recombination are the primary sources of genetic variation. To better understand the evolution of genetic variation, it is crucial to comprehensively investigate the processes involving mutation accumulation and recombination. In this study, we performed mutation accumulation experiments on four heterozygous diploid yeast species in the Saccharomycodaceae family to determine spontaneous mutation rates, mutation spectra, and losses of heterozygosity (LOH). We observed substantial variation in mutation rates and mutation spectra. We also observed high LOH rates (1.65-11.07×10-6 events per heterozygous site per cell division). Biases in spontaneous mutation and LOH together with selection ultimately shape the variable genome-wide nucleotide landscape in yeast species.
Original language | English (US) |
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Pages (from-to) | 3118-3130 |
Number of pages | 13 |
Journal | Molecular biology and evolution |
Volume | 37 |
Issue number | 11 |
DOIs | |
State | Published - Nov 1 2020 |
Keywords
- Base substitutions
- Diploid genome
- GC content
- LOH
- Mutation rate
- Mutational spectrum
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Molecular Biology
- Genetics