Fiber reinforcement in precast tunnel segments is quite attractive since it reduces the cost and labor associated with the fabrication process while it improves the post-cracking behavior considerably. Among the benefits, one can address improvements in handling, fatigue, impact resistance and durability while reducing the crack widths significantly. Flexural tests of full-scale precast tunnel segments are conducted using a newly developed testing laboratory for large structural testing. Using the closed-form material properties obtained from the flexural beam specimens, the response of full sections is predicted and the test results are compared with the full-scale test results obtained on the testing facility. Using the proposed methodologies, one can develop proper material and structural models for the accurate design of tunnel segments due to their unique design characteristics.