Vaccine synergy with virus-like particle and immune complex platforms for delivery of human papillomavirus L2 antigen

Andrew G. Diamos, Dalia Larios, Lauren Brown, Jacquelyn Kilbourne, Hyun Soon Kim, Divyasha Saxena, Kenneth E. Palmer, Hugh S. Mason

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Diverse HPV subtypes are responsible for considerable disease burden worldwide, necessitating safe, cheap, and effective vaccines. The HPV minor capsid protein L2 is a promising candidate to create broadly protective HPV vaccines, though it is poorly immunogenic by itself. To create highly immunogenic and safe vaccine candidates targeting L2, we employed a plant-based recombinant protein expression system to produce two different vaccine candidates: L2 displayed on the surface of hepatitis B core (HBc) virus-like particles (VLPs) or L2 genetically fused to an immunoglobulin capable of forming recombinant immune complexes (RIC). Both vaccine candidates were potently immunogenic in mice, but were especially so when delivered together, generating very consistent and high antibody titers directed against HPV L2 (>1,000,000) that correlated with virus neutralization. These data indicate a novel immune response synergy upon co-delivery of VLP and RIC platforms, a strategy that can be adapted generally for many different antigens.

Original languageEnglish (US)
Pages (from-to)137-144
Number of pages8
JournalVaccine
Volume37
Issue number1
DOIs
StatePublished - Jan 3 2019

Keywords

  • Human papillomavirus
  • Minor capsid protein
  • Recombinant immune complex
  • Vaccine
  • Virus-like particle

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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