TY - JOUR
T1 - UV photolysis for relieved inhibition of sulfadiazine (SD) to biomass growth
AU - Pan, Shihui
AU - Yan, Ning
AU - Zhang, Yongming
AU - Rittmann, Bruce
N1 - Publisher Copyright:
© 2014 Springer-Verlag Berlin Heidelberg.
PY - 2015/3/22
Y1 - 2015/3/22
N2 - Abstract UV photolysis was used to relieve inhibition of biomass growth by sulfadiazine (SD), a broad-spectrum anti-microbial. To investigate the effects of SD on biomass growth, three substrates - glucose alone (G), glucose plus sulfadiazine (G+SD), and glucose plus photolyzed SD (G+PSD) - were used to culture the bacteria acclimated to glucose. The biomass was strongly inhibited when SD was added into the glucose solution, but inhibition was relieved to a significant degree when the SD was treated with UV irradiation as a pretreatment. The biomass growth kinetics were described well by the Monod model when glucose was used as a substrate alone, but the kinetics followed a hybrid Aiba model for non-competitive inhibition when SD was added to the solution. When photolyzed SD was added to glucose solution to replace original SD, the growth still followed Aiba inhibition, but inhibition was significantly relieved: the maximum specific growth rate (μ max) increased by 17%, and the Aiba inhibition concentration increased by 60%. Aniline, a major product of UV photolysis, supported the growth of the glucose-biodegrading bacteria. Thus, UV photolysis of SD significantly relieved inhibition by lowering the SD concentration and by generating a biodegradable product.
AB - Abstract UV photolysis was used to relieve inhibition of biomass growth by sulfadiazine (SD), a broad-spectrum anti-microbial. To investigate the effects of SD on biomass growth, three substrates - glucose alone (G), glucose plus sulfadiazine (G+SD), and glucose plus photolyzed SD (G+PSD) - were used to culture the bacteria acclimated to glucose. The biomass was strongly inhibited when SD was added into the glucose solution, but inhibition was relieved to a significant degree when the SD was treated with UV irradiation as a pretreatment. The biomass growth kinetics were described well by the Monod model when glucose was used as a substrate alone, but the kinetics followed a hybrid Aiba model for non-competitive inhibition when SD was added to the solution. When photolyzed SD was added to glucose solution to replace original SD, the growth still followed Aiba inhibition, but inhibition was significantly relieved: the maximum specific growth rate (μ max) increased by 17%, and the Aiba inhibition concentration increased by 60%. Aniline, a major product of UV photolysis, supported the growth of the glucose-biodegrading bacteria. Thus, UV photolysis of SD significantly relieved inhibition by lowering the SD concentration and by generating a biodegradable product.
KW - Inhibition
KW - Metabolism pathway
KW - Sulfadiazine
KW - Ultraviolet radiation
UR - http://www.scopus.com/inward/record.url?scp=84938231422&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84938231422&partnerID=8YFLogxK
U2 - 10.1007/s00449-014-1335-x
DO - 10.1007/s00449-014-1335-x
M3 - Article
C2 - 25533040
AN - SCOPUS:84938231422
SN - 1615-7591
VL - 38
SP - 911
EP - 915
JO - Bioprocess and biosystems engineering
JF - Bioprocess and biosystems engineering
IS - 5
M1 - 1335
ER -