Using spaceflight and spaceflight analogue culture for novel mechanistic insight into Salmonella pathogenesis

Jennifer Barrila, James W. Wilson, Anjali Soni, Jiseon Yang, C. Mark Ott, Cheryl Nickerson

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

As the first bacterial pathogen to be profiled for changes in virulence in response to either spaceflight or spaceflight analogue culture, Salmonella enterica serovar Typhimurium (S. Typhimurium) has served as a model organism for evaluating the potential of these environments to alter the pathogenesis-related characteristics of microbes. This chapter describes a series of progressive studies conducted with S. Typhimurium that have established the paradigm that the low fluid shear environment present during spaceflight and spaceflight analogue culture (as well as in the infected host in vivo) could alter the virulence, pathogenesis-related stress responses, and global gene expression profiles of microbial pathogens like Salmonella. The exciting discovery that these environments could reprogram S. Typhimurium in a unique manner, thereby leading to the identification of entire classes of microbial genes/proteins involved in host interactions not previously identified under conventional culture conditions, laid the foundation for these experiments to be conducted with other microbial pathogens. Follow-up studies with other pathogens ultimately unveiled evolutionarily conserved responses to the microgravity and microgravity analogue environments, including the RNA chaperone Hfq, thus demonstrating that bacteria were "hard-wired" to respond to these conditions. These findings have important implications for astronaut health and hold potential for development of novel strategies for treatment and prevention for the general public.

Original languageEnglish (US)
Title of host publicationEffect of Spaceflight and Spaceflight Analogue Culture on Human and Microbial Cells: Novel Insights into Disease Mechanisms
PublisherSpringer New York
Pages209-235
Number of pages27
ISBN (Electronic)9781493932771
ISBN (Print)9781493932764
DOIs
StatePublished - Jan 1 2016

Fingerprint

salmonella
Space Flight
pathogenesis
pathogens
Salmonella
virulence
analogs
Weightlessness
microgravity
Virulence
Microbial Genes
Astronauts
astronauts
Salmonella enterica
gene expression
microorganisms
Transcriptome
organisms
genes
bacteria

Keywords

  • Gene expression
  • Hfq
  • Low shear modeled microgravity
  • Microgravity
  • Physiological fluid shear
  • Rotating wall vessel bioreactor
  • Salmonella pathogenesis
  • Spaceflight
  • Virulence

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology and Microbiology(all)
  • Physics and Astronomy(all)

Cite this

Barrila, J., Wilson, J. W., Soni, A., Yang, J., Ott, C. M., & Nickerson, C. (2016). Using spaceflight and spaceflight analogue culture for novel mechanistic insight into Salmonella pathogenesis. In Effect of Spaceflight and Spaceflight Analogue Culture on Human and Microbial Cells: Novel Insights into Disease Mechanisms (pp. 209-235). Springer New York. https://doi.org/10.1007/978-1-4939-3277-1_11

Using spaceflight and spaceflight analogue culture for novel mechanistic insight into Salmonella pathogenesis. / Barrila, Jennifer; Wilson, James W.; Soni, Anjali; Yang, Jiseon; Ott, C. Mark; Nickerson, Cheryl.

Effect of Spaceflight and Spaceflight Analogue Culture on Human and Microbial Cells: Novel Insights into Disease Mechanisms. Springer New York, 2016. p. 209-235.

Research output: Chapter in Book/Report/Conference proceedingChapter

Barrila, J, Wilson, JW, Soni, A, Yang, J, Ott, CM & Nickerson, C 2016, Using spaceflight and spaceflight analogue culture for novel mechanistic insight into Salmonella pathogenesis. in Effect of Spaceflight and Spaceflight Analogue Culture on Human and Microbial Cells: Novel Insights into Disease Mechanisms. Springer New York, pp. 209-235. https://doi.org/10.1007/978-1-4939-3277-1_11
Barrila J, Wilson JW, Soni A, Yang J, Ott CM, Nickerson C. Using spaceflight and spaceflight analogue culture for novel mechanistic insight into Salmonella pathogenesis. In Effect of Spaceflight and Spaceflight Analogue Culture on Human and Microbial Cells: Novel Insights into Disease Mechanisms. Springer New York. 2016. p. 209-235 https://doi.org/10.1007/978-1-4939-3277-1_11
Barrila, Jennifer ; Wilson, James W. ; Soni, Anjali ; Yang, Jiseon ; Ott, C. Mark ; Nickerson, Cheryl. / Using spaceflight and spaceflight analogue culture for novel mechanistic insight into Salmonella pathogenesis. Effect of Spaceflight and Spaceflight Analogue Culture on Human and Microbial Cells: Novel Insights into Disease Mechanisms. Springer New York, 2016. pp. 209-235
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