Abstract
Electrophoretic exclusion, a novel separations technique that differentiates species in bulk solution using the opposing forces of electrophoretic velocity and hydrodynamic flow, has been adapted to a microscale device. Proof-of-principle experiments indicate that the device was able to exclude small particles (1 μm polystyrene microspheres) and fluorescent dye molecules (rhodamine 123) from the entrance of a channel. Additionally, differentiation of the rhodamine 123 and polystyrene spheres was demonstrated. The current studies focus on the direct observation of the electrophoretic exclusion behavior on a microchip.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1227-1235 |
| Number of pages | 9 |
| Journal | Electrophoresis |
| Volume | 33 |
| Issue number | 8 |
| DOIs | |
| State | Published - Apr 2012 |
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Keywords
- Capillary electrophoresis
- Electrophoretic exclusion
- Microscale
- Separations
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
Cite this
Using electrophoretic exclusion to manipulate small molecules and particles on a microdevice. / Kenyon, Stacy M.; Weiss, Noah G.; Hayes, Mark.
In: Electrophoresis, Vol. 33, No. 8, 04.2012, p. 1227-1235.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Using electrophoretic exclusion to manipulate small molecules and particles on a microdevice
AU - Kenyon, Stacy M.
AU - Weiss, Noah G.
AU - Hayes, Mark
PY - 2012/4
Y1 - 2012/4
N2 - Electrophoretic exclusion, a novel separations technique that differentiates species in bulk solution using the opposing forces of electrophoretic velocity and hydrodynamic flow, has been adapted to a microscale device. Proof-of-principle experiments indicate that the device was able to exclude small particles (1 μm polystyrene microspheres) and fluorescent dye molecules (rhodamine 123) from the entrance of a channel. Additionally, differentiation of the rhodamine 123 and polystyrene spheres was demonstrated. The current studies focus on the direct observation of the electrophoretic exclusion behavior on a microchip.
AB - Electrophoretic exclusion, a novel separations technique that differentiates species in bulk solution using the opposing forces of electrophoretic velocity and hydrodynamic flow, has been adapted to a microscale device. Proof-of-principle experiments indicate that the device was able to exclude small particles (1 μm polystyrene microspheres) and fluorescent dye molecules (rhodamine 123) from the entrance of a channel. Additionally, differentiation of the rhodamine 123 and polystyrene spheres was demonstrated. The current studies focus on the direct observation of the electrophoretic exclusion behavior on a microchip.
KW - Capillary electrophoresis
KW - Electrophoretic exclusion
KW - Microscale
KW - Separations
UR - http://www.scopus.com/inward/record.url?scp=84861009983&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861009983&partnerID=8YFLogxK
U2 - 10.1002/elps.201100622
DO - 10.1002/elps.201100622
M3 - Article
VL - 33
SP - 1227
EP - 1235
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 8
ER -