Abstract
Electrophoretic exclusion, a novel separations technique that differentiates species in bulk solution using the opposing forces of electrophoretic velocity and hydrodynamic flow, has been adapted to a microscale device. Proof-of-principle experiments indicate that the device was able to exclude small particles (1 μm polystyrene microspheres) and fluorescent dye molecules (rhodamine 123) from the entrance of a channel. Additionally, differentiation of the rhodamine 123 and polystyrene spheres was demonstrated. The current studies focus on the direct observation of the electrophoretic exclusion behavior on a microchip.
Original language | English (US) |
---|---|
Pages (from-to) | 1227-1235 |
Number of pages | 9 |
Journal | Electrophoresis |
Volume | 33 |
Issue number | 8 |
DOIs | |
State | Published - Apr 2012 |
Keywords
- Capillary electrophoresis
- Electrophoretic exclusion
- Microscale
- Separations
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry