Using electrophoretic exclusion to manipulate small molecules and particles on a microdevice

Stacy M. Kenyon, Noah G. Weiss, Mark Hayes

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Electrophoretic exclusion, a novel separations technique that differentiates species in bulk solution using the opposing forces of electrophoretic velocity and hydrodynamic flow, has been adapted to a microscale device. Proof-of-principle experiments indicate that the device was able to exclude small particles (1 μm polystyrene microspheres) and fluorescent dye molecules (rhodamine 123) from the entrance of a channel. Additionally, differentiation of the rhodamine 123 and polystyrene spheres was demonstrated. The current studies focus on the direct observation of the electrophoretic exclusion behavior on a microchip.

Original languageEnglish (US)
Pages (from-to)1227-1235
Number of pages9
JournalElectrophoresis
Volume33
Issue number8
DOIs
StatePublished - Apr 1 2012

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Keywords

  • Capillary electrophoresis
  • Electrophoretic exclusion
  • Microscale
  • Separations

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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