TY - JOUR
T1 - Use of UV-irradiated bacteriophage T6 to kill extracellular bacteria in tissue culture infectivity assays
AU - Shaw, Denise R.
AU - Maurelli, Anthony T.
AU - Goguen, Jon D.
AU - Straley, Susan C.
AU - Curtiss, Roy
PY - 1983/1/14
Y1 - 1983/1/14
N2 - We have utilized 'lysis from without' mediated by UV-inactivated bacteriophage T6 to eliminate extracellular bacteria in experiments measuring the internalization, intracellular survival and replication of Yersinia pestis within mouse peritoneal macrophages and of Shigella flexneri within a human intestinal epithelial cell line. The technique we describe has the following characteristics: (a) bacterial killing is complete within 15 min at 37°C, with a > 103-fold reduction in colony-forming units (CFU); (b) bacteria within cultured mammalian cells are protected from killing by UV-inactivated T6; (c) the mammalian cells are not observably affected by exposure to UV-inactivated T6. This technique has several advantages over the use of antibiotics to eliminate extracellular bacteria and is potentially widely applicable in studies of the interactions between pathogenic bacteria and host phagocytic cells as well as other target tissues.
AB - We have utilized 'lysis from without' mediated by UV-inactivated bacteriophage T6 to eliminate extracellular bacteria in experiments measuring the internalization, intracellular survival and replication of Yersinia pestis within mouse peritoneal macrophages and of Shigella flexneri within a human intestinal epithelial cell line. The technique we describe has the following characteristics: (a) bacterial killing is complete within 15 min at 37°C, with a > 103-fold reduction in colony-forming units (CFU); (b) bacteria within cultured mammalian cells are protected from killing by UV-inactivated T6; (c) the mammalian cells are not observably affected by exposure to UV-inactivated T6. This technique has several advantages over the use of antibiotics to eliminate extracellular bacteria and is potentially widely applicable in studies of the interactions between pathogenic bacteria and host phagocytic cells as well as other target tissues.
KW - bacteria-phagocyte interactions
KW - bactericidal assays
KW - in vitro infectivity assays
KW - intracellular bacterial pathogens
KW - phagocytosis
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U2 - 10.1016/0022-1759(83)90050-9
DO - 10.1016/0022-1759(83)90050-9
M3 - Article
C2 - 6338113
AN - SCOPUS:0020665261
VL - 56
SP - 75
EP - 83
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1
ER -