TY - JOUR
T1 - Use of salivary biomarkers in biobehavioral research
T2 - Cotton-based sample collection methods can interfere with salivary immunoassay results
AU - Shirtcliff, Elizabeth A.
AU - Granger, Douglas A.
AU - Schwartz, Eve
AU - Curran, Mary J.
N1 - Funding Information:
This study was supported by The Pennsylvania State University Behavioral Endocrinology Laboratory. Technical support was provided by Jodi Heaton and Thema Nicholson. We gratefully acknowledge the contribution of reagents by Rusty Nicar and Prasanna Harihar of Diagnostic Systems Laboratories (Webster, TX).
PY - 2001
Y1 - 2001
N2 - In a series of studies, we evaluated the susceptibility of immunoassays for saliva biomarkers to interference effects caused by cotton materials used to absorb saliva during sample collection. Salivary assay results for testosterone, DHEA, progesterone, and estradiol are artificially high, and for sIgA artificially low, when samples are collected using cotton absorbent materials. In contrast, results for salivary cortisol, DHEA-S, and cotinine are not affected by the use of cotton collection methods. The order of individual results from samples collected using cotton versus no-cotton methods for certain markers is not conserved, suggesting that for some biomarkers this collection method can be a significant source of unsystematic error. It was shown, using DHEA as an example, that the cotton interference effect is of sufficient magnitude to attenuate the association between serum and saliva levels. Awareness of this issue is critical to ensure measurement validity in future studies and analyses of archived samples collected using cotton materials. Copyright (C) 2001 Elsevier Science Ltd.
AB - In a series of studies, we evaluated the susceptibility of immunoassays for saliva biomarkers to interference effects caused by cotton materials used to absorb saliva during sample collection. Salivary assay results for testosterone, DHEA, progesterone, and estradiol are artificially high, and for sIgA artificially low, when samples are collected using cotton absorbent materials. In contrast, results for salivary cortisol, DHEA-S, and cotinine are not affected by the use of cotton collection methods. The order of individual results from samples collected using cotton versus no-cotton methods for certain markers is not conserved, suggesting that for some biomarkers this collection method can be a significant source of unsystematic error. It was shown, using DHEA as an example, that the cotton interference effect is of sufficient magnitude to attenuate the association between serum and saliva levels. Awareness of this issue is critical to ensure measurement validity in future studies and analyses of archived samples collected using cotton materials. Copyright (C) 2001 Elsevier Science Ltd.
KW - Cotinine
KW - Hormones
KW - Immunoassay
KW - Saliva
KW - Sample collection
KW - sIgA
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U2 - 10.1016/S0306-4530(00)00042-1
DO - 10.1016/S0306-4530(00)00042-1
M3 - Article
C2 - 11087962
AN - SCOPUS:0035219242
VL - 26
SP - 165
EP - 173
JO - Psychoneuroendocrinology
JF - Psychoneuroendocrinology
SN - 0306-4530
IS - 2
ER -