Use of ribosomal RNA-based molecular probes for characterization of complex microbial communities in anaerobic biofilms

L. Raskin, R. I. Amann, L. K. Poulsen, Bruce Rittmann, D. A. Stahl

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The use of ribosomal RNA (rRNA) probe technology for the characterization of complex microbial communities is reviewed and illustrated by discussing the results of a long-term study of four anaerobic fixed-bed biofilm reactors. Two distinct approaches were used to characterize the microbial community structure in these biofilm reactors. The first used a collection of phylogenetically defined oligonucleotide rRNA probes for methanogens and sulfate-reducing bacteria (SRB) to quantify their populations. Population abundance was linked to the functional behavior of the biofilm reactor community by determining the effluent concentrations of the substrates, intermediates, and final products of microbial metabolism. This analysis indicated that the presence of SRB (especially Desulfovibrio-species) was not dependent upon the presence of sulfate. Methanobacteriales-species were the major competitors for hydrogen with these SRB in the absence of sulfate. The second approach involved selective amplification, cloning, sequencing, and whole cell hybridization to identify, visualize, and isolate a biofilm community member (strain PT-2). Subsequently, it was determined that the growth rate of strain PT-2 was significantly higher in young biofilms than in established biofilms.

Original languageEnglish (US)
Pages (from-to)261-272
Number of pages12
JournalWater Science and Technology
Volume31
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Biofilms
biofilm
microbial community
probe
sulfate-reducing bacterium
Bacteria
Methanogens
sulfate
Oligonucleotides
Cloning
Metabolism
Amplification
RNA
Effluents
amplification
community structure
metabolism
Sulfates
hydrogen
effluent

Keywords

  • Biofilms
  • Hybridization
  • Methanogens
  • Oligonucleotide probes
  • Ribosomal RNA
  • Sulfate-reducing bacteria

ASJC Scopus subject areas

  • Water Science and Technology

Cite this

Use of ribosomal RNA-based molecular probes for characterization of complex microbial communities in anaerobic biofilms. / Raskin, L.; Amann, R. I.; Poulsen, L. K.; Rittmann, Bruce; Stahl, D. A.

In: Water Science and Technology, Vol. 31, No. 1, 1995, p. 261-272.

Research output: Contribution to journalArticle

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