Use of cationized ferritin nanoparticles to measure renal glomerular microstructure with MRI

Kevin M. Bennett, Scott C. Beeman, Edwin J. Baldelomar, Min Zhang, Teresa Wu, Bradley D. Hann, John F. Bertram, Jennifer R. Charlton

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

Magnetic resonance imaging (MRI) is becoming important for whole-kidney assessment of glomerular morphology, both in vivo and ex vivo. MRI-based renal morphological measurements can be made in intact organs and allow direct measurements of every perfused glomerulus. Cationic ferritin (CF) is used as a superparamagnetic contrast agent for MRI. CF binds to the glomerular basement membrane after intravenous injection, allowing direct, whole-kidney measurements of glomerular number, volume, and volume distribution. Here we describe the production, testing, and use of CF as an MRI contrast agent for quantitative glomerular morphology in intact mouse, rat, and human kidneys.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages67-79
Number of pages13
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1397
ISSN (Print)1064-3745

Keywords

  • Cationic ferritin
  • Chronic kidney disease
  • Contrast agent
  • Glomerulus
  • Kidney
  • Magnetic resonance imaging
  • Morphology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Bennett, K. M., Beeman, S. C., Baldelomar, E. J., Zhang, M., Wu, T., Hann, B. D., Bertram, J. F., & Charlton, J. R. (2016). Use of cationized ferritin nanoparticles to measure renal glomerular microstructure with MRI. In Methods in Molecular Biology (pp. 67-79). (Methods in Molecular Biology; Vol. 1397). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3353-2_7