Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model

Joseph F. Georges, Xiaowei Liu, Jennifer Eschbacher, Joshua Nichols, Michael A. Mooney, Anna Joy, Robert F. Spetzler, Burt G. Feuerstein, Mark C. Preul, Trent Anderson, Hao Yan, Peter Nakaji

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Improved tools for providing specific intraoperative diagnoses could improve patient care. In neurosurgery, intraoperatively differentiating non-operative lesions such as CNS B-cell lymphoma from operative lesions can be challenging, often necessitating immunohistochemical (IHC) procedures which require up to 24-48 hours. Here, we evaluate the feasibility of generating rapid ex vivo specific labeling using a novel lymphoma-specific fluorescent switchable aptamer. Our B-cell lymphoma-specific switchable aptamer produced only low-level fluorescence in its unbound conformation and generated an 8-fold increase in fluorescence once bound to its target on CD20-positive lymphoma cells. The aptamer demonstrated strong binding to B-cell lymphoma cells within 15 minutes of incubation as observed by flow cytometry. We applied the switchable aptamer to ex vivo xenograft tissue harboring B-cell lymphoma and astrocytoma, and within one hour specific visual identification of lymphoma was routinely possible. In this proof-of-concept study in human cell culture and orthotopic xenografts, we conclude that a fluorescent switchable aptamer can provide rapid and specific labeling of B-cell lymphoma, and that developing aptamer-based labeling approaches could simplify tissue staining and drastically reduce time to histopathological diagnoses compared with IHC-based methods. We propose that switchable aptamers could enhance expeditious, accurate intraoperative decision-making.

Original languageEnglish (US)
Article numbere0123607
JournalPLoS One
Volume10
Issue number4
DOIs
StatePublished - Apr 15 2015

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Neurology
B-Cell Lymphoma
lymphoma
Heterografts
central nervous system
Lymphoma
Central Nervous System
Cells
B-lymphocytes
Labeling
Fluorescence
Tissue
Neurosurgery
lesions (animal)
Flow cytometry
Astrocytoma
Cell culture
fluorescence
Conformations
Decision Making

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model. / Georges, Joseph F.; Liu, Xiaowei; Eschbacher, Jennifer; Nichols, Joshua; Mooney, Michael A.; Joy, Anna; Spetzler, Robert F.; Feuerstein, Burt G.; Preul, Mark C.; Anderson, Trent; Yan, Hao; Nakaji, Peter.

In: PLoS One, Vol. 10, No. 4, e0123607, 15.04.2015.

Research output: Contribution to journalArticle

Georges, JF, Liu, X, Eschbacher, J, Nichols, J, Mooney, MA, Joy, A, Spetzler, RF, Feuerstein, BG, Preul, MC, Anderson, T, Yan, H & Nakaji, P 2015, 'Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model', PLoS One, vol. 10, no. 4, e0123607. https://doi.org/10.1371/journal.pone.0123607
Georges, Joseph F. ; Liu, Xiaowei ; Eschbacher, Jennifer ; Nichols, Joshua ; Mooney, Michael A. ; Joy, Anna ; Spetzler, Robert F. ; Feuerstein, Burt G. ; Preul, Mark C. ; Anderson, Trent ; Yan, Hao ; Nakaji, Peter. / Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model. In: PLoS One. 2015 ; Vol. 10, No. 4.
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