Urinary Biomarkers of Renal Disease in Dogs with X-Linked Hereditary Nephropathy

M. B. Nabity, G. E. Lees, R. Cianciolo, M. M. Boggess, J. M. Steiner, J. S. Suchodolski

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Background: Sensitive and specific biomarkers for early tubulointerstitial injury are lacking. Hypothesis: The excretion of certain urinary proteins will correlate with the state of renal injury in dogs with chronic kidney disease. Animals: Twenty-five male colony dogs affected with X-linked hereditary nephropathy (XLHN) and 19 unaffected male littermates were evaluated. Methods: Retrospective analysis of urine samples collected every 2-4 weeks was performed. Urine proteins evaluated were retinol binding protein (uRBP/c), β2-microglobulin (uB2M), N-acetyl-β-d-glucosaminidase (uNAG/c), neutrophil gelatinase-associated lipocalin (uNGAL/c), and immunoglobulin G (uIgG/c). Results were correlated with serum creatinine concentration (sCr), glomerular filtration rate (GFR), urine protein: creatinine ratio, and histopathologic analysis of serial renal biopsies. Analytical validation was performed for all assays; uNAG stability was evaluated. Results: All urinary biomarkers distinguished affected dogs from unaffected dogs early in their disease process, increasing during early and midstages of disease. uRBP/c correlated most strongly with conventional measures of disease severity, including increasing sCr (r = 0.89), decreasing GFR (r = -0.77), and interstitial fibrosis (r = 0.80), P < .001. However, multivariate analysis revealed age, sCr, uIgG/c, and uB2M, but not uRBP/c, as significant independent predictors of GFR (P < .05). Conclusions and Clinical Importance: All urinary biomarkers were elevated before sCr increased, but typically after proteinuria developed in dogs with progressive glomerular disease because of XLHN. uRBP/c measurement might be promising as a noninvasive tool for diagnosis and monitoring of tubular injury and dysfunction in dogs.

Original languageEnglish (US)
Pages (from-to)282-293
Number of pages12
JournalJournal of Veterinary Internal Medicine
Volume26
Issue number2
DOIs
StatePublished - Mar 2012
Externally publishedYes

Fingerprint

Dog Diseases
kidney diseases
biomarkers
creatinine
Biomarkers
Dogs
Creatinine
Kidney
glomerular filtration rate
dogs
Glomerular Filtration Rate
Urine
Serum
Wounds and Injuries
urine
kidneys
Hexosaminidases
Retinol-Binding Proteins
retinol-binding protein
Proteins

Keywords

  • β2-microglobulin
  • N-acetyl-β-d-glucosaminidase
  • Neutrophil gelatinase-associated lipocalin
  • Retinol binding protein

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Urinary Biomarkers of Renal Disease in Dogs with X-Linked Hereditary Nephropathy. / Nabity, M. B.; Lees, G. E.; Cianciolo, R.; Boggess, M. M.; Steiner, J. M.; Suchodolski, J. S.

In: Journal of Veterinary Internal Medicine, Vol. 26, No. 2, 03.2012, p. 282-293.

Research output: Contribution to journalArticle

Nabity, M. B. ; Lees, G. E. ; Cianciolo, R. ; Boggess, M. M. ; Steiner, J. M. ; Suchodolski, J. S. / Urinary Biomarkers of Renal Disease in Dogs with X-Linked Hereditary Nephropathy. In: Journal of Veterinary Internal Medicine. 2012 ; Vol. 26, No. 2. pp. 282-293.
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AU - Steiner, J. M.

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