Urinary Biomarkers of Renal Disease in Dogs with X-Linked Hereditary Nephropathy

M. B. Nabity, G. E. Lees, R. Cianciolo, M. M. Boggess, J. M. Steiner, J. S. Suchodolski

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Background: Sensitive and specific biomarkers for early tubulointerstitial injury are lacking. Hypothesis: The excretion of certain urinary proteins will correlate with the state of renal injury in dogs with chronic kidney disease. Animals: Twenty-five male colony dogs affected with X-linked hereditary nephropathy (XLHN) and 19 unaffected male littermates were evaluated. Methods: Retrospective analysis of urine samples collected every 2-4 weeks was performed. Urine proteins evaluated were retinol binding protein (uRBP/c), β2-microglobulin (uB2M), N-acetyl-β-d-glucosaminidase (uNAG/c), neutrophil gelatinase-associated lipocalin (uNGAL/c), and immunoglobulin G (uIgG/c). Results were correlated with serum creatinine concentration (sCr), glomerular filtration rate (GFR), urine protein: creatinine ratio, and histopathologic analysis of serial renal biopsies. Analytical validation was performed for all assays; uNAG stability was evaluated. Results: All urinary biomarkers distinguished affected dogs from unaffected dogs early in their disease process, increasing during early and midstages of disease. uRBP/c correlated most strongly with conventional measures of disease severity, including increasing sCr (r = 0.89), decreasing GFR (r = -0.77), and interstitial fibrosis (r = 0.80), P < .001. However, multivariate analysis revealed age, sCr, uIgG/c, and uB2M, but not uRBP/c, as significant independent predictors of GFR (P < .05). Conclusions and Clinical Importance: All urinary biomarkers were elevated before sCr increased, but typically after proteinuria developed in dogs with progressive glomerular disease because of XLHN. uRBP/c measurement might be promising as a noninvasive tool for diagnosis and monitoring of tubular injury and dysfunction in dogs.

Original languageEnglish (US)
Pages (from-to)282-293
Number of pages12
JournalJournal of Veterinary Internal Medicine
Volume26
Issue number2
DOIs
StatePublished - Mar 2012
Externally publishedYes

Keywords

  • N-acetyl-β-d-glucosaminidase
  • Neutrophil gelatinase-associated lipocalin
  • Retinol binding protein
  • β2-microglobulin

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint

Dive into the research topics of 'Urinary Biomarkers of Renal Disease in Dogs with X-Linked Hereditary Nephropathy'. Together they form a unique fingerprint.

Cite this