Abstract

We evaluated a hydrogen-based membrane biofilm reactor (MBfR) for its capacity to reduce and remove hexavalent uranium [U(VI)] from water. After a startup period that allowed slow-growing U(VI) reducers to form biofilms, the MBfR successfully achieved and maintained 94-95% U(VI) removal over 8 months when the U surface loading was 6-11e- mEq/m2-day. The MBfR biofilm was capable of self-recovery after a disturbance due to oxygen exposure. Nanocrystalline UO2 aggregates and amorphous U precipitates were associated with vegetative cells and apparently mature spores that accumulated in the biofilm matrix. Despite inoculation with a concentrated suspension of Desulfovibrio vulgaris, this bacterium was not present in the U(VI)-reducing biofilm. Instead, the most abundant group in the biofilm community contained U(VI) reducers in the Rhodocyclaceae family when U(VI) was the only electron acceptor. When sulfate was present, the community dramatically shifted to the Clostridiaceae family, which included spores that were potentially involved in U(VI) reduction.

Original languageEnglish (US)
Pages (from-to)255-264
Number of pages10
JournalWater Research
Volume64
DOIs
StatePublished - Nov 1 2014

Fingerprint

Biofilms
Uranium
biofilm
microbial community
uranium
membrane
Membranes
spore
removal
reactor
inoculation
Precipitates
Bacteria
hydrogen
sulfate
disturbance
Recovery
electron
Hydrogen
oxygen

Keywords

  • Bicarbonate
  • MBfR
  • Spore
  • Sulfate
  • U removal
  • Uraninite

ASJC Scopus subject areas

  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution
  • Ecological Modeling

Cite this

Uranium removal and microbial community in a H2-based membrane biofilm reactor. / Zhou, Chen; Ontiveros-Valencia, Aura; Cornette de Saint Cyr, Louis; Zevin, Alexander S.; Carey, Sara E.; Krajmalnik-Brown, Rosa; Rittmann, Bruce.

In: Water Research, Vol. 64, 01.11.2014, p. 255-264.

Research output: Contribution to journalArticle

Zhou C, Ontiveros-Valencia A, Cornette de Saint Cyr L, Zevin AS, Carey SE, Krajmalnik-Brown R et al. Uranium removal and microbial community in a H2-based membrane biofilm reactor. Water Research. 2014 Nov 1;64:255-264. https://doi.org/10.1016/j.watres.2014.07.013
Zhou, Chen ; Ontiveros-Valencia, Aura ; Cornette de Saint Cyr, Louis ; Zevin, Alexander S. ; Carey, Sara E. ; Krajmalnik-Brown, Rosa ; Rittmann, Bruce. / Uranium removal and microbial community in a H2-based membrane biofilm reactor. In: Water Research. 2014 ; Vol. 64. pp. 255-264.
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