Unusual suppression properties of lysogens containing derivatives of ϕ80 psu3+

Sheldon I. Feinstein, Sidney Altman

Research output: Contribution to journalArticlepeer-review

Abstract

Nonsense codon suppressing lysogens of E. coli have been made using φ{symbol}80 psu3+-A2 and φ{symbol}80 psuoc+-A2, heat-sensitive amber and ochre suppressing derivatives, respectively, of bacteriophage φ{symbol}80. The various lysogens selected differ in strength of suppression as well as in heat sensitivity of suppressor function. Heat-resistant derivatives, some still carrying the A2 mutation, can be selected from the heat sensitive parents. Mapping expreiments indicate that the φ{symbol}80 derivatives integrate at the tyrTV locus, which contains two copies of tRNA1Tyr. The origin of the various suppressor phenotypes appears to be related to the great variety of distinctive recombination events possible either between the incoming tRNA1Tyrgene and the host copies, or among the three copies of this gene in the lysogens.

Original languageEnglish (US)
Pages (from-to)347-353
Number of pages7
JournalMGG Molecular & General Genetics
Volume187
Issue number2
DOIs
StatePublished - Oct 1982
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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