Unraveling the spatial distribution of immunoglobulins, enzymes, and polyelectrolytes within layer-by-layer self-assembled multilayers. Ellipsometric studies

N. F. Ferreyra, Erica Forzani, M. López Teijelo, L. Coche-Guérente, P. Labbé

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Abstract

The ellipsometric characterization of a layer-by-layer electrostatically self-assembled multilayer of polyphenol oxidase and alkaline phosphatase with the polycation poly(dimethyldiallylammonium chloride) built on an immunologie layer formed by immunoglobulin G (IgG) and glucose oxidase-conjugated anti-IgG (IgG-GOD) on glassy carbon is reported. The step-by-step evolution of the ψ-Δ ellipsometric angles was followed during film growth. Two optical models, named the three-layer film model and reorganization film model, were employed and found suitable for ellipsometric data interpretation. A comparative analysis of film optical properties, film thickness, and ellipsometric mass assessed from both models is also presented.

Original languageEnglish (US)
Pages (from-to)8931-8938
Number of pages8
JournalLangmuir
Volume22
Issue number21
DOIs
Publication statusPublished - Oct 10 2006

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ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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