Unraveling the spatial distribution of immunoglobulins, enzymes, and polyelectrolytes within layer-by-layer self-assembled multilayers. Ellipsometric studies

N. F. Ferreyra, Erica Forzani, M. López Teijelo, L. Coche-Guérente, P. Labbé

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The ellipsometric characterization of a layer-by-layer electrostatically self-assembled multilayer of polyphenol oxidase and alkaline phosphatase with the polycation poly(dimethyldiallylammonium chloride) built on an immunologie layer formed by immunoglobulin G (IgG) and glucose oxidase-conjugated anti-IgG (IgG-GOD) on glassy carbon is reported. The step-by-step evolution of the ψ-Δ ellipsometric angles was followed during film growth. Two optical models, named the three-layer film model and reorganization film model, were employed and found suitable for ellipsometric data interpretation. A comparative analysis of film optical properties, film thickness, and ellipsometric mass assessed from both models is also presented.

Original languageEnglish (US)
Pages (from-to)8931-8938
Number of pages8
JournalLangmuir
Volume22
Issue number21
DOIs
StatePublished - Oct 10 2006

Fingerprint

Polyelectrolytes
Spatial distribution
enzymes
Immunoglobulins
spatial distribution
Multilayers
Enzymes
Immunoglobulin G
oxidase
Catechol Oxidase
Glucose Oxidase
Glucose oxidase
phosphatases
glassy carbon
Phosphatases
Glassy carbon
Film growth
glucose
Alkaline Phosphatase
Film thickness

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Unraveling the spatial distribution of immunoglobulins, enzymes, and polyelectrolytes within layer-by-layer self-assembled multilayers. Ellipsometric studies. / Ferreyra, N. F.; Forzani, Erica; López Teijelo, M.; Coche-Guérente, L.; Labbé, P.

In: Langmuir, Vol. 22, No. 21, 10.10.2006, p. 8931-8938.

Research output: Contribution to journalArticle

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