Unraveling the spatial distribution of immunoglobulins, enzymes, and polyelectrolytes within layer-by-layer self-assembled multilayers. Ellipsometric studies

N. F. Ferreyra; Erica Forzani; M. López Teijelo; L. Coche-Guérente; P. Labbé

doi:10.1021/la061173q

Unraveling the spatial distribution of immunoglobulins, enzymes, and polyelectrolytes within layer-by-layer self-assembled multilayers. Ellipsometric studies

N. F. Ferreyra, Erica Forzani, M. López Teijelo, L. Coche-Guérente, P. Labbé

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The ellipsometric characterization of a layer-by-layer electrostatically self-assembled multilayer of polyphenol oxidase and alkaline phosphatase with the polycation poly(dimethyldiallylammonium chloride) built on an immunologie layer formed by immunoglobulin G (IgG) and glucose oxidase-conjugated anti-IgG (IgG-GOD) on glassy carbon is reported. The step-by-step evolution of the ψ-Δ ellipsometric angles was followed during film growth. Two optical models, named the three-layer film model and reorganization film model, were employed and found suitable for ellipsometric data interpretation. A comparative analysis of film optical properties, film thickness, and ellipsometric mass assessed from both models is also presented.

Original language	English (US)
Pages (from-to)	8931-8938
Number of pages	8
Journal	Langmuir
Volume	22
Issue number	21
DOIs	https://doi.org/10.1021/la061173q
State	Published - Oct 10 2006

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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abstract = "The ellipsometric characterization of a layer-by-layer electrostatically self-assembled multilayer of polyphenol oxidase and alkaline phosphatase with the polycation poly(dimethyldiallylammonium chloride) built on an immunologie layer formed by immunoglobulin G (IgG) and glucose oxidase-conjugated anti-IgG (IgG-GOD) on glassy carbon is reported. The step-by-step evolution of the ψ-Δ ellipsometric angles was followed during film growth. Two optical models, named the three-layer film model and reorganization film model, were employed and found suitable for ellipsometric data interpretation. A comparative analysis of film optical properties, film thickness, and ellipsometric mass assessed from both models is also presented.",

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T1 - Unraveling the spatial distribution of immunoglobulins, enzymes, and polyelectrolytes within layer-by-layer self-assembled multilayers. Ellipsometric studies

AU - Ferreyra, N. F.

AU - Forzani, Erica

AU - López Teijelo, M.

AU - Coche-Guérente, L.

AU - Labbé, P.

PY - 2006/10/10

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AB - The ellipsometric characterization of a layer-by-layer electrostatically self-assembled multilayer of polyphenol oxidase and alkaline phosphatase with the polycation poly(dimethyldiallylammonium chloride) built on an immunologie layer formed by immunoglobulin G (IgG) and glucose oxidase-conjugated anti-IgG (IgG-GOD) on glassy carbon is reported. The step-by-step evolution of the ψ-Δ ellipsometric angles was followed during film growth. Two optical models, named the three-layer film model and reorganization film model, were employed and found suitable for ellipsometric data interpretation. A comparative analysis of film optical properties, film thickness, and ellipsometric mass assessed from both models is also presented.

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Unraveling the spatial distribution of immunoglobulins, enzymes, and polyelectrolytes within layer-by-layer self-assembled multilayers. Ellipsometric studies

Abstract

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