TY - JOUR
T1 - Unexpected mitochondrial genome diversity revealed by targeted single-cell genomics of heterotrophic flagellated protists
AU - Wideman, Jeremy G.
AU - Monier, Adam
AU - Rodríguez-Martínez, Raquel
AU - Leonard, Guy
AU - Cook, Emily
AU - Poirier, Camille
AU - Maguire, Finlay
AU - Milner, David S.
AU - Irwin, Nicholas A.T.
AU - Moore, Karen
AU - Santoro, Alyson E.
AU - Keeling, Patrick J.
AU - Worden, Alexandra Z.
AU - Richards, Thomas A.
N1 - Publisher Copyright:
© 2019, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Most eukaryotic microbial diversity is uncultivated, under-studied and lacks nuclear genome data. Mitochondrial genome sampling is more comprehensive, but many phylogenetically important groups remain unsampled. Here, using a single-cell sorting approach combining tubulin-specific labelling with photopigment exclusion, we sorted flagellated heterotrophic unicellular eukaryotes from Pacific Ocean samples. We recovered 206 single amplified genomes, predominantly from underrepresented branches on the tree of life. Seventy single amplified genomes contained unique mitochondrial contigs, including 21 complete or near-complete mitochondrial genomes from formerly under-sampled phylogenetic branches, including telonemids, katablepharids, cercozoans and marine stramenopiles, effectively doubling the number of available samples of heterotrophic flagellate mitochondrial genomes. Collectively, these data identify a dynamic history of mitochondrial genome evolution including intron gain and loss, extensive patterns of genetic code variation and complex patterns of gene loss. Surprisingly, we found that stramenopile mitochondrial content is highly plastic, resembling patterns of variation previously observed only in plants.
AB - Most eukaryotic microbial diversity is uncultivated, under-studied and lacks nuclear genome data. Mitochondrial genome sampling is more comprehensive, but many phylogenetically important groups remain unsampled. Here, using a single-cell sorting approach combining tubulin-specific labelling with photopigment exclusion, we sorted flagellated heterotrophic unicellular eukaryotes from Pacific Ocean samples. We recovered 206 single amplified genomes, predominantly from underrepresented branches on the tree of life. Seventy single amplified genomes contained unique mitochondrial contigs, including 21 complete or near-complete mitochondrial genomes from formerly under-sampled phylogenetic branches, including telonemids, katablepharids, cercozoans and marine stramenopiles, effectively doubling the number of available samples of heterotrophic flagellate mitochondrial genomes. Collectively, these data identify a dynamic history of mitochondrial genome evolution including intron gain and loss, extensive patterns of genetic code variation and complex patterns of gene loss. Surprisingly, we found that stramenopile mitochondrial content is highly plastic, resembling patterns of variation previously observed only in plants.
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U2 - 10.1038/s41564-019-0605-4
DO - 10.1038/s41564-019-0605-4
M3 - Article
C2 - 31768028
AN - SCOPUS:85075439179
SN - 2058-5276
VL - 5
SP - 154
EP - 165
JO - Nature Microbiology
JF - Nature Microbiology
IS - 1
ER -