Uncovering system-specific stress signatures in primate teeth with multimodal imaging

Christine Austin, Tanya M. Smith, Ramin M Z Farahani, Katherine Hinde, Elizabeth A. Carter, Joonsup Lee, Peter A. Lay, Brendan J. Kennedy, Babak Sarrafpour, Rosalind J. Wright, Robert O. Wright, Manish Arora

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Early life stress can disrupt development and negatively impact long-Term health trajectories. Reconstructing histories of early life exposure to external stressors is hampered by the absence of retrospective time-specific biomarkers. Defects in tooth enamel have been used to reconstruct stress but the methods used are subjective and do not identify the specific biological systems impacted by external stressors. Here we show that external physical and social stressors impart biochemical signatures in primate teeth that can be retrieved to objectively reconstruct the timing of early life developmental disruptions. Using teeth from captive macaques, we uncovered elemental imprints specific to disruptions of skeletal growth, including major disruptions in body weight trajectory and moderate to severe illnesses. Discrete increases in heat shock protein-70 expression in dentine coincided with elemental signatures, confirming that elemental signals were associated with activation of stress-related pathways. To overcome limitations of conventional light-microscopic analysis, we used high resolution Raman microspectral imaging to identify structural and compositional alterations in enamel and dentine that coincided with elemental signatures and with detailed medical and behavioural data. Integrating these objective biochemical markers with temporal mapping of teeth enables the retrospective study of early life developmental disruptions and their ensuing health sequelae.

Original languageEnglish (US)
Article number18802
JournalScientific Reports
Volume6
DOIs
StatePublished - Jan 4 2016

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Multimodal Imaging
Primates
Tooth
Dentin
Dental Enamel
Biomarkers
HSP70 Heat-Shock Proteins
Health
Macaca
Psychological Stress
Retrospective Studies
Body Weight
Light
Growth

ASJC Scopus subject areas

  • General

Cite this

Austin, C., Smith, T. M., Farahani, R. M. Z., Hinde, K., Carter, E. A., Lee, J., ... Arora, M. (2016). Uncovering system-specific stress signatures in primate teeth with multimodal imaging. Scientific Reports, 6, [18802]. https://doi.org/10.1038/srep18802

Uncovering system-specific stress signatures in primate teeth with multimodal imaging. / Austin, Christine; Smith, Tanya M.; Farahani, Ramin M Z; Hinde, Katherine; Carter, Elizabeth A.; Lee, Joonsup; Lay, Peter A.; Kennedy, Brendan J.; Sarrafpour, Babak; Wright, Rosalind J.; Wright, Robert O.; Arora, Manish.

In: Scientific Reports, Vol. 6, 18802, 04.01.2016.

Research output: Contribution to journalArticle

Austin, C, Smith, TM, Farahani, RMZ, Hinde, K, Carter, EA, Lee, J, Lay, PA, Kennedy, BJ, Sarrafpour, B, Wright, RJ, Wright, RO & Arora, M 2016, 'Uncovering system-specific stress signatures in primate teeth with multimodal imaging', Scientific Reports, vol. 6, 18802. https://doi.org/10.1038/srep18802
Austin, Christine ; Smith, Tanya M. ; Farahani, Ramin M Z ; Hinde, Katherine ; Carter, Elizabeth A. ; Lee, Joonsup ; Lay, Peter A. ; Kennedy, Brendan J. ; Sarrafpour, Babak ; Wright, Rosalind J. ; Wright, Robert O. ; Arora, Manish. / Uncovering system-specific stress signatures in primate teeth with multimodal imaging. In: Scientific Reports. 2016 ; Vol. 6.
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