Ultrasound induced permeabilization of cell membranes as a therapy for cytotoxic neuronal edema

A. B. Hensley, Jitendran Muthuswamy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

One of the consequences of ischemic injury to neurons is cytotoxic edema, which has several undesirable consequences including an increase in the intra-cranial pressure. This project tests the hypothesis that pressure gradients induced by ultrasonic waves can be used to permeabilize the cell membrane leading to changes in ionic gradients and an efflux of water reversing the swelling effect. In a N1E mouse neuroblastoma cell culture model of lactacidosis, swelling was induced by lowering the pH of the medium. Flow cytometry measurements indicate dramatic increased scatter intensity accompanied by an increase in the complexity of the dendritic arbors at pH of 4.0. Upon sonication at 1 MHz and an intensity of 0.1 W/cm 2 for 3 minutes, the top 30% of scatter intensity values dropped by 18% accompanied by a decrease in complexity and size. In a similar model, swelling was also induced in N2A mouse neuroblastoma where the pH of the medium was dropped to 4.0. Upon sonication at 1 MHz and an intensity of 0.1 W/cm 2 for 3 minutes a reduction in swelling was imaged captured using a camera and an inverted microscope. In conclusion, ultra-sound induced permeabilization may be a potentially promising approach for treating cytotoxic neuronal edema.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages2123-2124
Number of pages2
Volume3
StatePublished - 2002
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Other

OtherProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS)
CountryUnited States
CityHouston, TX
Period10/23/0210/26/02

Keywords

  • Cytotoxic edema
  • Neurons
  • Permeabilization
  • Ultrasound

ASJC Scopus subject areas

  • Bioengineering

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    Hensley, A. B., & Muthuswamy, J. (2002). Ultrasound induced permeabilization of cell membranes as a therapy for cytotoxic neuronal edema. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 3, pp. 2123-2124)