Ultrafast excitation dynamics of low energy pigments in reconstituted peripheral light-harvesting complexes of photosystem I

Alexander N. Melkozernov, Su Lin, Volkmar H R Schmid, Harald Paulsen, Gregory W. Schmidt, Robert E. Blankenship

Research output: Contribution to journal › Article

28 Citations (Scopus)

Abstract

Ultrafast dynamics of a reconstituted Lhca4 subunit from the peripheral LHCI-730 antenna of photosystem I of higher plants were probed by femtosecond absorption spectroscopy at 77 K. Intramonomeric energy transfer from chlorophyll (Chl) b to Chl a and energy equilibration between Chl a molecules observed on the subpicosecond time scale are largely similar to subpicosecond energy equilibration processes within LHCII monomers. However, a 5 ps equilibration process in Lhca4 involves unique low energy Chls in LHCI absorbing at 705 nm. These pigments localize the excitation both in the Lhca4 subunit and in LHCI-730 heterodimers. An additional 30-50 ps equilibration process involving red pigments of Lhca4 in the heterodimer, observed by transient absorption and picosecond fluorescence spectroscopy, was ascribed to intersubunit energy transfer. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)89-92
Number of pages4
JournalFEBS Letters
Volume471
Issue number1
DOIs
StatePublished - Apr 7 2000

Fingerprint

Photosystem I Protein Complex
Energy Transfer
Pigments
Energy transfer
Light
Fluorescence Spectrometry
Fluorescence spectroscopy
Absorption spectroscopy
Spectrum Analysis
Monomers
Antennas
Molecules
chlorophyll a
chlorophyll b

Keywords

  • Antenna
  • Excitation energy transfer
  • LHCI-730 heterodimer
  • Light-harvesting complex
  • Photosystem I
  • Time-resolved spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ultrafast excitation dynamics of low energy pigments in reconstituted peripheral light-harvesting complexes of photosystem I. / Melkozernov, Alexander N.; Lin, Su; Schmid, Volkmar H R; Paulsen, Harald; Schmidt, Gregory W.; Blankenship, Robert E.

In: FEBS Letters, Vol. 471, No. 1, 07.04.2000, p. 89-92.

Research output: Contribution to journal › Article

Melkozernov, AN, Lin, S, Schmid, VHR, Paulsen, H, Schmidt, GW & Blankenship, RE 2000, 'Ultrafast excitation dynamics of low energy pigments in reconstituted peripheral light-harvesting complexes of photosystem I', FEBS Letters, vol. 471, no. 1, pp. 89-92. https://doi.org/10.1016/S0014-5793(00)01370-3
Melkozernov AN, Lin S, Schmid VHR, Paulsen H, Schmidt GW, Blankenship RE. Ultrafast excitation dynamics of low energy pigments in reconstituted peripheral light-harvesting complexes of photosystem I. FEBS Letters. 2000 Apr 7;471(1):89-92. https://doi.org/10.1016/S0014-5793(00)01370-3
Melkozernov, Alexander N. ; Lin, Su ; Schmid, Volkmar H R ; Paulsen, Harald ; Schmidt, Gregory W. ; Blankenship, Robert E. / Ultrafast excitation dynamics of low energy pigments in reconstituted peripheral light-harvesting complexes of photosystem I. In: FEBS Letters. 2000 ; Vol. 471, No. 1. pp. 89-92.
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AU - Melkozernov, Alexander N.

AU - Lin, Su

AU - Schmid, Volkmar H R

AU - Paulsen, Harald

AU - Schmidt, Gregory W.

AU - Blankenship, Robert E.

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N2 - Ultrafast dynamics of a reconstituted Lhca4 subunit from the peripheral LHCI-730 antenna of photosystem I of higher plants were probed by femtosecond absorption spectroscopy at 77 K. Intramonomeric energy transfer from chlorophyll (Chl) b to Chl a and energy equilibration between Chl a molecules observed on the subpicosecond time scale are largely similar to subpicosecond energy equilibration processes within LHCII monomers. However, a 5 ps equilibration process in Lhca4 involves unique low energy Chls in LHCI absorbing at 705 nm. These pigments localize the excitation both in the Lhca4 subunit and in LHCI-730 heterodimers. An additional 30-50 ps equilibration process involving red pigments of Lhca4 in the heterodimer, observed by transient absorption and picosecond fluorescence spectroscopy, was ascribed to intersubunit energy transfer. Copyright (C) 2000 Federation of European Biochemical Societies.

AB - Ultrafast dynamics of a reconstituted Lhca4 subunit from the peripheral LHCI-730 antenna of photosystem I of higher plants were probed by femtosecond absorption spectroscopy at 77 K. Intramonomeric energy transfer from chlorophyll (Chl) b to Chl a and energy equilibration between Chl a molecules observed on the subpicosecond time scale are largely similar to subpicosecond energy equilibration processes within LHCII monomers. However, a 5 ps equilibration process in Lhca4 involves unique low energy Chls in LHCI absorbing at 705 nm. These pigments localize the excitation both in the Lhca4 subunit and in LHCI-730 heterodimers. An additional 30-50 ps equilibration process involving red pigments of Lhca4 in the heterodimer, observed by transient absorption and picosecond fluorescence spectroscopy, was ascribed to intersubunit energy transfer. Copyright (C) 2000 Federation of European Biochemical Societies.

KW - Antenna

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KW - LHCI-730 heterodimer

KW - Light-harvesting complex

KW - Photosystem I

KW - Time-resolved spectroscopy

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