TY - GEN
T1 - Ultra-high ratio dilution microfluidic system for single strand DNA isolation
AU - Christen, Jennifer Blain
AU - Iglehart, Brian
AU - Pouliquen, Philippe
PY - 2008
Y1 - 2008
N2 - We present a system comprised of a PDMS microfluidic device and an injection system for dilution of DNA derived from whole blood to a single strand. We discuss two microfluidic architectures for ultra-high ratio dilution (1:1000 or more) as well as the necessity to move beyond previously reported dilution architectures. We describe a custom injection system used both for fabrication and operation of the system. The system is operated by placing syringes, one filled with water and one filled with DNA extracted from whole blood, into the injection system. This system fixes and plunges the syringes simultaneously to introduce both solutions into the microfluidic channels that produce the ultra-high ratio dilutions. The most significant constraint on the design is that the system is designed for fabrication, characterization and use in a clinical setting.
AB - We present a system comprised of a PDMS microfluidic device and an injection system for dilution of DNA derived from whole blood to a single strand. We discuss two microfluidic architectures for ultra-high ratio dilution (1:1000 or more) as well as the necessity to move beyond previously reported dilution architectures. We describe a custom injection system used both for fabrication and operation of the system. The system is operated by placing syringes, one filled with water and one filled with DNA extracted from whole blood, into the injection system. This system fixes and plunges the syringes simultaneously to introduce both solutions into the microfluidic channels that produce the ultra-high ratio dilutions. The most significant constraint on the design is that the system is designed for fabrication, characterization and use in a clinical setting.
UR - http://www.scopus.com/inward/record.url?scp=51749083240&partnerID=8YFLogxK
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U2 - 10.1109/ISCAS.2008.4541794
DO - 10.1109/ISCAS.2008.4541794
M3 - Conference contribution
AN - SCOPUS:51749083240
SN - 9781424416844
T3 - Proceedings - IEEE International Symposium on Circuits and Systems
SP - 1822
EP - 1825
BT - 2008 IEEE International Symposium on Circuits and Systems, ISCAS 2008
T2 - 2008 IEEE International Symposium on Circuits and Systems, ISCAS 2008
Y2 - 18 May 2008 through 21 May 2008
ER -