Ultra-high ratio dilution microfluidic system for single strand DNA isolation

Jennifer Blain Christen, Brian Iglehart, Philippe Pouliquen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a system comprised of a PDMS microfluidic device and an injection system for dilution of DNA derived from whole blood to a single strand. We discuss two microfluidic architectures for ultra-high ratio dilution (1:1000 or more) as well as the necessity to move beyond previously reported dilution architectures. We describe a custom injection system used both for fabrication and operation of the system. The system is operated by placing syringes, one filled with water and one filled with DNA extracted from whole blood, into the injection system. This system fixes and plunges the syringes simultaneously to introduce both solutions into the microfluidic channels that produce the ultra-high ratio dilutions. The most significant constraint on the design is that the system is designed for fabrication, characterization and use in a clinical setting.

Original languageEnglish (US)
Title of host publication2008 IEEE International Symposium on Circuits and Systems, ISCAS 2008
Pages1822-1825
Number of pages4
DOIs
StatePublished - Sep 19 2008
Externally publishedYes
Event2008 IEEE International Symposium on Circuits and Systems, ISCAS 2008 - Seattle, WA, United States
Duration: May 18 2008May 21 2008

Publication series

NameProceedings - IEEE International Symposium on Circuits and Systems
ISSN (Print)0271-4310

Other

Other2008 IEEE International Symposium on Circuits and Systems, ISCAS 2008
CountryUnited States
CitySeattle, WA
Period5/18/085/21/08

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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    Christen, J. B., Iglehart, B., & Pouliquen, P. (2008). Ultra-high ratio dilution microfluidic system for single strand DNA isolation. In 2008 IEEE International Symposium on Circuits and Systems, ISCAS 2008 (pp. 1822-1825). [4541794] (Proceedings - IEEE International Symposium on Circuits and Systems). https://doi.org/10.1109/ISCAS.2008.4541794