Two-tank suspended growth process for accelerating the detoxification kinetics of hydrocarbons requiring initial monooxygenation reactions

Elizabeth P. Dahlen, Bruce E. Rittmann

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

An experimental evaluation demonstrated that suspended growth systems operated in a two-tank accelerator/aerator configuration significantly increased the overall removal rates for phenol and 2,4-dichlorophenol (2,4-DCP), aromatic hydrocarbons that require initial monooxygenations. The accelerator tank is a small volume that receives the influent and recycled biomass. It has a high ratio of electron donor (BOD) to electron acceptor (02). Biomass in the accelerator should be enriched in reduced nicotinamide adenine dinucleotide (NADH + H+) and have a very high specific growth rate, conditions that should accelerate the kinetics of monooxygenation reactions. For the more slowly degraded 2,4-DCP, the average percentage removal increased from 74% to 93%, even though the volume of the two-tank system was smaller than that of the one-tank system in most experiments. The average volumetric and biomass-specific removal rates increased by 50% and 100%, respectively, in the two-tank system, compared to a one-tank system. The greatest enhancement in 2,4-DCP removal occurred when the accelerator tank comprised approximately 20% of the system volume. Biomass in the accelerator tank was significantly enriched in NADH + H+ when its dissolved oxygen (DO) concentration was below 0.25 mg/L, a situation having a high ratio of donor to acceptor. The accelerator biomass had its highest NADH + H+ content for the experiments that had the highest rate of 2,4-DCP removal. Biomass in the accelerator also had a much higher specific growth rate than in the aerator or the system overall, and the specific growth rate in the accelerator was inversely correlated to the accelerator volume.

Original languageEnglish (US)
Pages (from-to)101-116
Number of pages16
JournalBiodegradation
Volume13
Issue number2
DOIs
StatePublished - Nov 13 2002
Externally publishedYes

Keywords

  • Dichlorophenol
  • Dissolved oxygen
  • Monooxygenation
  • Nicotinamide adenine dinucleotide
  • Phenolics
  • Specific growth rate
  • Suspended growth

ASJC Scopus subject areas

  • Environmental Engineering
  • Microbiology
  • Bioengineering
  • Environmental Chemistry
  • Pollution

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