TY - JOUR
T1 - Two-stage cultivation of Nannochloropsis oculata for lipid production using reversible alkaline flocculation
AU - Aléman-Nava, Gibran Sidney
AU - Muylaert, Koenraad
AU - Cuellar Bermudez, Sara Paulina
AU - Depraetere, Orily
AU - Rittmann, Bruce
AU - Parra-Saldívar, Roberto
AU - Vandamme, Dries
N1 - Funding Information:
D. V. is a postdoctoral Researcher funded by the Research Foundation – Flanders (FWO) (12D8914N). O.D. is a postdoctoral Researcher funded by the KU Leuven – Postdoctoral mandate IF (PDM/15/109). The authors would like to thank Coimbra Group Programme for Latin America for the scholarship provided to Gibrán Alemán-Nava during his research stay at KU Leuven.
Publisher Copyright:
© 2016 Elsevier Ltd
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Two-stage cultivation for microalgae biomass is a promising strategy to boost lipid accumulation and productivity. Most of the currently described processes use energy-intensive centrifugation for cell separation after the first cultivation stage. This laboratory study evaluated alkaline flocculation as low-cost alternative separation method to harvest Nannochloropsis oculata prior to cultivation in the second nutrient-depleted cultivation stage. Biomass concentration over time and the maximum quantum yield of photosystem II expressed as Fv:Fm ratio showed identical patterns for both harvesting methods in both stages. The composition of total lipids, carbohydrates, and protein was similar for biomass harvested via alkaline flocculation or centrifugation. Likewise, both harvest methods yielded the same increase in total lipid content, to 40% within the first 2 days of the nutrient-depleted stage, with an enrichment in C16 fatty acid methyl esters. Centrifugation can therefore be replaced with alkaline flocculation to harvest Nannochloropsis oculata after the first cultivation stage.
AB - Two-stage cultivation for microalgae biomass is a promising strategy to boost lipid accumulation and productivity. Most of the currently described processes use energy-intensive centrifugation for cell separation after the first cultivation stage. This laboratory study evaluated alkaline flocculation as low-cost alternative separation method to harvest Nannochloropsis oculata prior to cultivation in the second nutrient-depleted cultivation stage. Biomass concentration over time and the maximum quantum yield of photosystem II expressed as Fv:Fm ratio showed identical patterns for both harvesting methods in both stages. The composition of total lipids, carbohydrates, and protein was similar for biomass harvested via alkaline flocculation or centrifugation. Likewise, both harvest methods yielded the same increase in total lipid content, to 40% within the first 2 days of the nutrient-depleted stage, with an enrichment in C16 fatty acid methyl esters. Centrifugation can therefore be replaced with alkaline flocculation to harvest Nannochloropsis oculata after the first cultivation stage.
KW - Algae
KW - Flocculation
KW - Lipids
KW - Nutrient starvation
KW - Separation
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U2 - 10.1016/j.biortech.2016.11.121
DO - 10.1016/j.biortech.2016.11.121
M3 - Article
C2 - 27988475
AN - SCOPUS:85006751461
SN - 0960-8524
VL - 226
SP - 18
EP - 23
JO - Bioresource Technology
JF - Bioresource Technology
ER -