Two-stage cultivation of Nannochloropsis oculata for lipid production using reversible alkaline flocculation

Gibran Sidney Aléman-Nava, Koenraad Muylaert, Sara Paulina Cuellar Bermudez, Orily Depraetere, Bruce Rittmann, Roberto Parra-Saldívar, Dries Vandamme

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Two-stage cultivation for microalgae biomass is a promising strategy to boost lipid accumulation and productivity. Most of the currently described processes use energy-intensive centrifugation for cell separation after the first cultivation stage. This laboratory study evaluated alkaline flocculation as low-cost alternative separation method to harvest Nannochloropsis oculata prior to cultivation in the second nutrient-depleted cultivation stage. Biomass concentration over time and the maximum quantum yield of photosystem II expressed as Fv:Fm ratio showed identical patterns for both harvesting methods in both stages. The composition of total lipids, carbohydrates, and protein was similar for biomass harvested via alkaline flocculation or centrifugation. Likewise, both harvest methods yielded the same increase in total lipid content, to 40% within the first 2 days of the nutrient-depleted stage, with an enrichment in C16 fatty acid methyl esters. Centrifugation can therefore be replaced with alkaline flocculation to harvest Nannochloropsis oculata after the first cultivation stage.

Original languageEnglish (US)
Pages (from-to)18-23
Number of pages6
JournalBioresource Technology
Volume226
DOIs
StatePublished - Feb 1 2017

Fingerprint

Flocculation
flocculation
Lipids
lipid
Centrifugation
Biomass
Nutrients
biomass
Photosystem II Protein Complex
nutrient
Quantum yield
Carbohydrates
energy use
Fatty acids
ester
carbohydrate
Esters
Fatty Acids
fatty acid
Productivity

Keywords

  • Algae
  • Flocculation
  • Lipids
  • Nutrient starvation
  • Separation

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Aléman-Nava, G. S., Muylaert, K., Cuellar Bermudez, S. P., Depraetere, O., Rittmann, B., Parra-Saldívar, R., & Vandamme, D. (2017). Two-stage cultivation of Nannochloropsis oculata for lipid production using reversible alkaline flocculation. Bioresource Technology, 226, 18-23. https://doi.org/10.1016/j.biortech.2016.11.121

Two-stage cultivation of Nannochloropsis oculata for lipid production using reversible alkaline flocculation. / Aléman-Nava, Gibran Sidney; Muylaert, Koenraad; Cuellar Bermudez, Sara Paulina; Depraetere, Orily; Rittmann, Bruce; Parra-Saldívar, Roberto; Vandamme, Dries.

In: Bioresource Technology, Vol. 226, 01.02.2017, p. 18-23.

Research output: Contribution to journalArticle

Aléman-Nava, GS, Muylaert, K, Cuellar Bermudez, SP, Depraetere, O, Rittmann, B, Parra-Saldívar, R & Vandamme, D 2017, 'Two-stage cultivation of Nannochloropsis oculata for lipid production using reversible alkaline flocculation', Bioresource Technology, vol. 226, pp. 18-23. https://doi.org/10.1016/j.biortech.2016.11.121
Aléman-Nava, Gibran Sidney ; Muylaert, Koenraad ; Cuellar Bermudez, Sara Paulina ; Depraetere, Orily ; Rittmann, Bruce ; Parra-Saldívar, Roberto ; Vandamme, Dries. / Two-stage cultivation of Nannochloropsis oculata for lipid production using reversible alkaline flocculation. In: Bioresource Technology. 2017 ; Vol. 226. pp. 18-23.
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