Abstract

This paper reports a two-phase mini-column separator that depletes Immunoglobulin G (IgG) and separates α-fetoProtein (AFP) and fibrinogen. A mixture of IgG, fibrinogen, and AFP goes through the two-phase separator, where IgG is depleted by an affinity mini-column using Protein A/G agarose beads in the first phase. In the second phase, AFP is separated from fibrinogen by Size Exclusion Chromatography (SEC). The IgG depletion and SEC separation results are confirmed by evaluating fluorescent intensity of the eluents. Red (IgG), green (AFP), and blue (fibrinogen) intensities show that over 99.5% of IgG is depleted and, in 10 min, the second phase SEC minicolumn separates AFP (1 μg/mL) from fibrinogen-rich solution (10 μg/mL) with 2.78 resolution and 0.0056 mm plate height.

Original languageEnglish (US)
Title of host publicationTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages104-107
Number of pages4
DOIs
StatePublished - 2009
EventTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States
Duration: Jun 21 2009Jun 25 2009

Other

OtherTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
CountryUnited States
CityDenver, CO
Period6/21/096/25/09

Fingerprint

Size exclusion chromatography
Separators
Proteins

Keywords

  • Plasma
  • Sample preparation
  • Separator
  • Size exclusion chromatography

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Yang, Y., & Chae, J. (2009). Two-phase mini-separator to separate alpha-fetoprotein from fibrinogen and immunoglobulin. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 104-107). [5285552] https://doi.org/10.1109/SENSOR.2009.5285552

Two-phase mini-separator to separate alpha-fetoprotein from fibrinogen and immunoglobulin. / Yang, Yongmo; Chae, Junseok.

TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 104-107 5285552.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yang, Y & Chae, J 2009, Two-phase mini-separator to separate alpha-fetoprotein from fibrinogen and immunoglobulin. in TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems., 5285552, pp. 104-107, TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems, Denver, CO, United States, 6/21/09. https://doi.org/10.1109/SENSOR.2009.5285552
Yang Y, Chae J. Two-phase mini-separator to separate alpha-fetoprotein from fibrinogen and immunoglobulin. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 104-107. 5285552 https://doi.org/10.1109/SENSOR.2009.5285552
Yang, Yongmo ; Chae, Junseok. / Two-phase mini-separator to separate alpha-fetoprotein from fibrinogen and immunoglobulin. TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. pp. 104-107
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abstract = "This paper reports a two-phase mini-column separator that depletes Immunoglobulin G (IgG) and separates α-fetoProtein (AFP) and fibrinogen. A mixture of IgG, fibrinogen, and AFP goes through the two-phase separator, where IgG is depleted by an affinity mini-column using Protein A/G agarose beads in the first phase. In the second phase, AFP is separated from fibrinogen by Size Exclusion Chromatography (SEC). The IgG depletion and SEC separation results are confirmed by evaluating fluorescent intensity of the eluents. Red (IgG), green (AFP), and blue (fibrinogen) intensities show that over 99.5{\%} of IgG is depleted and, in 10 min, the second phase SEC minicolumn separates AFP (1 μg/mL) from fibrinogen-rich solution (10 μg/mL) with 2.78 resolution and 0.0056 mm plate height.",
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