TY - JOUR
T1 - Two equilibration pools of chlorophylls in the Photosystem I core antenna of Chlamydomonas reinhardtii
AU - Gibasiewicz, Krzysztof
AU - Ramesh, V. M.
AU - Lin, Su
AU - Redding, Kevin
AU - Woodbury, Neal
AU - Webber, Andrew
N1 - Funding Information:
Acknowledgments We are thankful to Prof. A. Freiberg for a valuable discussion. This work was supported by the DOE. K.R. acknowledges support from an NSF CAREER award (MCB-0347935). K.G. gratefully acknowledges financial support from the Polish government (scientific project no 1 P03B 003 29).
PY - 2007/4
Y1 - 2007/4
N2 - Femtosecond transient absorption spectroscopy was applied for a comparative study of excitation decay in several different Photosystem I (PSI) core preparations from the green alga Chlamydomonas reinhardtii. For PSI cores with a fully interconnected network of chlorophylls, the excitation energy was equilibrated over a pool of chlorophylls absorbing at ∼683 nm, independent of excitation wavelength [Gibasiewicz et al. J Phys Chem B 105:11498-11506, 2001; J Phys Chem B 106:6322-6330, 2002]. In preparations with impaired connectivity between chlorophylls, we have found that the spectrum of chlorophylls connected to the reaction center (i.e., with ∼20 ps decay time) over which the excitation is equilibrated becomes excitation-wavelength- dependent. Excitation at 670 nm is finally equilibrated over chlorophylls absorbing at ∼675 nm, whereas excitation at 695 nm or 700 nm is equilibrated over chlorophylls absorbing at ∼683 nm. This indicates that in the vicinity of the reaction center there are two spectrally different and spatially separated pools of chlorophylls that are equally capable of effective excitation energy transfer to the reaction center. We propose that they are related to the two groups of central PSI core chlorophylls lying on the opposite sides of reaction center.
AB - Femtosecond transient absorption spectroscopy was applied for a comparative study of excitation decay in several different Photosystem I (PSI) core preparations from the green alga Chlamydomonas reinhardtii. For PSI cores with a fully interconnected network of chlorophylls, the excitation energy was equilibrated over a pool of chlorophylls absorbing at ∼683 nm, independent of excitation wavelength [Gibasiewicz et al. J Phys Chem B 105:11498-11506, 2001; J Phys Chem B 106:6322-6330, 2002]. In preparations with impaired connectivity between chlorophylls, we have found that the spectrum of chlorophylls connected to the reaction center (i.e., with ∼20 ps decay time) over which the excitation is equilibrated becomes excitation-wavelength- dependent. Excitation at 670 nm is finally equilibrated over chlorophylls absorbing at ∼675 nm, whereas excitation at 695 nm or 700 nm is equilibrated over chlorophylls absorbing at ∼683 nm. This indicates that in the vicinity of the reaction center there are two spectrally different and spatially separated pools of chlorophylls that are equally capable of effective excitation energy transfer to the reaction center. We propose that they are related to the two groups of central PSI core chlorophylls lying on the opposite sides of reaction center.
KW - Antenna
KW - Chlorophylls
KW - Excitation energy transfer
KW - Femtosecond transient absorption
KW - Photosystem I
KW - Primary charge separation
KW - Reaction center
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U2 - 10.1007/s11120-006-9125-1
DO - 10.1007/s11120-006-9125-1
M3 - Article
C2 - 17611814
AN - SCOPUS:34547123801
SN - 0166-8595
VL - 92
SP - 55
EP - 63
JO - Photosynthesis research
JF - Photosynthesis research
IS - 1
ER -