Two-dimensional gel analysis of secreted proteins induced by interleukin-1β in rat astrocytes

Julia W. Chang, Donald A. Young, Paul Coleman, M. Kerry O'Banion

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Interleukin-1β (IL-1β) is a pro-inflammatory cytokine produced in the brain by endogenous microglial cells responding to injury. Levels of IL-1β are elevated in several neurodegenerative disorders, including Alzheimer's disease. IL-1β, which can act as a mitogen for astrocytes, also elicits the expression and secretion of multiple factors and paracrine 'second messengers' such as other cytokines, nerve growth factor, prostaglandins and nitric oxide that may in turn modulate neuronal and glial responses to injury. Utilizing giant, high-resolution two-dimensional gel electrophoresis, we have sought to more fully define the potential range of protein mediators that are secreted by astrocytes treated with IL-1β. In cultured rat astrocytes, we observe dramatic increases in the secretion of eight different protein species after 24 h of treatment with human recombinant IL-1β (1 U/ml). Seven of the proteins are also induced by tumor necrosis factor-α or basic fibroblast growth factor. Based on immunoprecipitation with specific antisera, we have identified three of these proteins as plasminogen activator inhibitor type-1, ceruloplasmin, and complement component C3. The identities of the other proteins, including the IL-1β-specific induction, are currently unknown. Characterization of these downstream modulators of IL-1β action complements gene-based approaches and will provide a better understanding of astrocyte responses to injury as well as markers for astrocyte activation in neurodegenerative diseases.

Original languageEnglish (US)
Pages (from-to)349-359
Number of pages11
JournalNeurochemistry International
Volume39
Issue number5-6
DOIs
StatePublished - Oct 9 2001
Externally publishedYes

Fingerprint

Interleukin-1
Astrocytes
Gels
Proteins
Neurodegenerative Diseases
Wounds and Injuries
Cytokines
Complement C3
Ceruloplasmin
Plasminogen Activator Inhibitor 1
Electrophoresis, Gel, Two-Dimensional
Second Messenger Systems
Nerve Growth Factor
Fibroblast Growth Factor 2
Mitogens
Immunoprecipitation
Neuroglia
Prostaglandins
Immune Sera
Alzheimer Disease

Keywords

  • Alzheimer's disease
  • Basic fibroblast growth factor
  • C3 complement component
  • Ceruloplasmin
  • CNS injury
  • Plasminogen activator inhibitor type-1
  • Tumor necrosis factor-α
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Two-dimensional gel analysis of secreted proteins induced by interleukin-1β in rat astrocytes. / Chang, Julia W.; Young, Donald A.; Coleman, Paul; O'Banion, M. Kerry.

In: Neurochemistry International, Vol. 39, No. 5-6, 09.10.2001, p. 349-359.

Research output: Contribution to journalArticle

Chang, Julia W. ; Young, Donald A. ; Coleman, Paul ; O'Banion, M. Kerry. / Two-dimensional gel analysis of secreted proteins induced by interleukin-1β in rat astrocytes. In: Neurochemistry International. 2001 ; Vol. 39, No. 5-6. pp. 349-359.
@article{d192f094472c40b4ada176c2e2de4193,
title = "Two-dimensional gel analysis of secreted proteins induced by interleukin-1β in rat astrocytes",
abstract = "Interleukin-1β (IL-1β) is a pro-inflammatory cytokine produced in the brain by endogenous microglial cells responding to injury. Levels of IL-1β are elevated in several neurodegenerative disorders, including Alzheimer's disease. IL-1β, which can act as a mitogen for astrocytes, also elicits the expression and secretion of multiple factors and paracrine 'second messengers' such as other cytokines, nerve growth factor, prostaglandins and nitric oxide that may in turn modulate neuronal and glial responses to injury. Utilizing giant, high-resolution two-dimensional gel electrophoresis, we have sought to more fully define the potential range of protein mediators that are secreted by astrocytes treated with IL-1β. In cultured rat astrocytes, we observe dramatic increases in the secretion of eight different protein species after 24 h of treatment with human recombinant IL-1β (1 U/ml). Seven of the proteins are also induced by tumor necrosis factor-α or basic fibroblast growth factor. Based on immunoprecipitation with specific antisera, we have identified three of these proteins as plasminogen activator inhibitor type-1, ceruloplasmin, and complement component C3. The identities of the other proteins, including the IL-1β-specific induction, are currently unknown. Characterization of these downstream modulators of IL-1β action complements gene-based approaches and will provide a better understanding of astrocyte responses to injury as well as markers for astrocyte activation in neurodegenerative diseases.",
keywords = "Alzheimer's disease, Basic fibroblast growth factor, C3 complement component, Ceruloplasmin, CNS injury, Plasminogen activator inhibitor type-1, Tumor necrosis factor-α, Two-dimensional gel electrophoresis",
author = "Chang, {Julia W.} and Young, {Donald A.} and Paul Coleman and O'Banion, {M. Kerry}",
year = "2001",
month = "10",
day = "9",
doi = "10.1016/S0197-0186(01)00042-0",
language = "English (US)",
volume = "39",
pages = "349--359",
journal = "Neurochemistry International",
issn = "0197-0186",
publisher = "Elsevier Limited",
number = "5-6",

}

TY - JOUR

T1 - Two-dimensional gel analysis of secreted proteins induced by interleukin-1β in rat astrocytes

AU - Chang, Julia W.

AU - Young, Donald A.

AU - Coleman, Paul

AU - O'Banion, M. Kerry

PY - 2001/10/9

Y1 - 2001/10/9

N2 - Interleukin-1β (IL-1β) is a pro-inflammatory cytokine produced in the brain by endogenous microglial cells responding to injury. Levels of IL-1β are elevated in several neurodegenerative disorders, including Alzheimer's disease. IL-1β, which can act as a mitogen for astrocytes, also elicits the expression and secretion of multiple factors and paracrine 'second messengers' such as other cytokines, nerve growth factor, prostaglandins and nitric oxide that may in turn modulate neuronal and glial responses to injury. Utilizing giant, high-resolution two-dimensional gel electrophoresis, we have sought to more fully define the potential range of protein mediators that are secreted by astrocytes treated with IL-1β. In cultured rat astrocytes, we observe dramatic increases in the secretion of eight different protein species after 24 h of treatment with human recombinant IL-1β (1 U/ml). Seven of the proteins are also induced by tumor necrosis factor-α or basic fibroblast growth factor. Based on immunoprecipitation with specific antisera, we have identified three of these proteins as plasminogen activator inhibitor type-1, ceruloplasmin, and complement component C3. The identities of the other proteins, including the IL-1β-specific induction, are currently unknown. Characterization of these downstream modulators of IL-1β action complements gene-based approaches and will provide a better understanding of astrocyte responses to injury as well as markers for astrocyte activation in neurodegenerative diseases.

AB - Interleukin-1β (IL-1β) is a pro-inflammatory cytokine produced in the brain by endogenous microglial cells responding to injury. Levels of IL-1β are elevated in several neurodegenerative disorders, including Alzheimer's disease. IL-1β, which can act as a mitogen for astrocytes, also elicits the expression and secretion of multiple factors and paracrine 'second messengers' such as other cytokines, nerve growth factor, prostaglandins and nitric oxide that may in turn modulate neuronal and glial responses to injury. Utilizing giant, high-resolution two-dimensional gel electrophoresis, we have sought to more fully define the potential range of protein mediators that are secreted by astrocytes treated with IL-1β. In cultured rat astrocytes, we observe dramatic increases in the secretion of eight different protein species after 24 h of treatment with human recombinant IL-1β (1 U/ml). Seven of the proteins are also induced by tumor necrosis factor-α or basic fibroblast growth factor. Based on immunoprecipitation with specific antisera, we have identified three of these proteins as plasminogen activator inhibitor type-1, ceruloplasmin, and complement component C3. The identities of the other proteins, including the IL-1β-specific induction, are currently unknown. Characterization of these downstream modulators of IL-1β action complements gene-based approaches and will provide a better understanding of astrocyte responses to injury as well as markers for astrocyte activation in neurodegenerative diseases.

KW - Alzheimer's disease

KW - Basic fibroblast growth factor

KW - C3 complement component

KW - Ceruloplasmin

KW - CNS injury

KW - Plasminogen activator inhibitor type-1

KW - Tumor necrosis factor-α

KW - Two-dimensional gel electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=0034802595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034802595&partnerID=8YFLogxK

U2 - 10.1016/S0197-0186(01)00042-0

DO - 10.1016/S0197-0186(01)00042-0

M3 - Article

C2 - 11578770

AN - SCOPUS:0034802595

VL - 39

SP - 349

EP - 359

JO - Neurochemistry International

JF - Neurochemistry International

SN - 0197-0186

IS - 5-6

ER -