Abstract
The foundational goal of structural DNA nanotechnology - the field that uses oligonucleotides as a molecular building block for the programmable self-assembly of nanostructured systems - was to use DNA to construct three-dimensional (3D) lattices for solving macromolecular structures. The programmable nature of DNA makes it an ideal system for rationally constructing self-assembled crystals and immobilizing guest molecules in a repeating 3D array through their specific stereospatial interactions with the scaffold. In this work, we have extended a previously described motif (4 × 5) by expanding the structure to a system that links four double-helical layers; we use a central weaving oligonucleotide containing a sequence of four six-base repeats (4 × 6), forming a matrix of layers that are organized and dictated by a series of Holliday junctions. In addition, we have assembled mirror image crystals (l-DNA) with the identical sequence that are completely resistant to nucleases. Bromine and selenium derivatives were obtained for the l- and d-DNA forms, respectively, allowing phase determination for both forms and solution of the resulting structures to 3.0 and 3.05 Å resolution. Both right- and left-handed forms crystallized in the trigonal space groups with mirror image 3-fold helical screw axes P32 and P31 for each motif, respectively. The structures reveal a highly organized array of discrete and well-defined cavities that are suitable for hosting guest molecules and allow us to dictate a priori the assembly of guest-DNA conjugates with a specified crystalline hand.
Original language | English (US) |
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Pages (from-to) | 11254-11260 |
Number of pages | 7 |
Journal | Journal of the American Chemical Society |
Volume | 139 |
Issue number | 32 |
DOIs | |
State | Published - Aug 16 2017 |
ASJC Scopus subject areas
- Catalysis
- General Chemistry
- Biochemistry
- Colloid and Surface Chemistry
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Dive into the research topics of 'Tuning the Cavity Size and Chirality of Self-Assembling 3D DNA Crystals'. Together they form a unique fingerprint.Datasets
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A self-assembling D-form DNA crystal lattice
Simmons, C. R. (Contributor), Zhang, F. (Contributor), MacCulloch, T. (Contributor), Fahmi, N. (Contributor), Stephanopoulos, N. (Contributor), Liu, Y. (Contributor), Seeman, N. C. (Contributor) & Yan, H. (Contributor), Protein Data Bank (PDB), Aug 2 2017
DOI: 10.2210/pdb5VY6, https://www.wwpdb.org/pdb?id=pdb_00005vy6
Dataset
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A self-assembling L-form DNA crystal lattice
Simmons, C. R. (Contributor), Zhang, F. (Contributor), MacCulloch, T. (Contributor), Fahmi, N. (Contributor), Stephanopoulos, N. (Contributor), Liu, Y. (Contributor), Seeman, N. C. (Contributor) & Yan, H. (Contributor), Protein Data Bank (PDB), Aug 9 2017
DOI: 10.2210/pdb5VY7, https://www.wwpdb.org/pdb?id=pdb_00005vy7
Dataset