Tuning myosin-driven sorting on cellular actin networks

Rizal Hariadi, Ruth Sommese, Sivaraj Sivaramakrishnan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Myosin V and VI are antagonistic motors that cohabit membrane vesicles in cells. A systematic study of their collective function, however, is lacking and forms the focus of this study. We functionally reconstitute a two-dimensional actin-myosin interface using myosin V and VI precisely patterned on DNA nanostructures, in combination with a model keratocyte actin meshwork. While scaffolds display solely unidirectional movement, their directional flux is modulated by both actin architecture and the structural properties of the myosin lever arm. This directional flux can be finely-tuned by the relative number of myosin V and VI motors on each scaffold. Pairing computation with experimental observations suggests that the ratio of motor stall forces is a key determinant of the observed competitive outcomes. Overall, our study demonstrates an elegant mechanism for sorting of membrane cargo using equally matched antagonistic motors, simply by modulating the relative number of engagement sites for each motor type.

Original languageEnglish (US)
Article numbere05472
JournaleLife
Volume2015
Issue number4
DOIs
StatePublished - Mar 4 2015
Externally publishedYes

Fingerprint

Myosin Type V
Myosins
Sorting
Actins
Tuning
Membranes
Scaffolds
Nanostructures
Fluxes
DNA
Structural properties
Display devices
myosin VI

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Tuning myosin-driven sorting on cellular actin networks. / Hariadi, Rizal; Sommese, Ruth; Sivaramakrishnan, Sivaraj.

In: eLife, Vol. 2015, No. 4, e05472, 04.03.2015.

Research output: Contribution to journalArticle

Hariadi, R, Sommese, R & Sivaramakrishnan, S 2015, 'Tuning myosin-driven sorting on cellular actin networks', eLife, vol. 2015, no. 4, e05472. https://doi.org/10.7554/eLife.05472
Hariadi, Rizal ; Sommese, Ruth ; Sivaramakrishnan, Sivaraj. / Tuning myosin-driven sorting on cellular actin networks. In: eLife. 2015 ; Vol. 2015, No. 4.
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