Tunable controlled release of bioactive SDF-1α via specific protein interactions within fibrin/nanoparticle composites

D. Dutta, C. Fauer, H. L. Mulleneux, Sarah Stabenfeldt

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The chemokine, stromal cell-derived factor 1α (SDF-1α), is a key regulator of the endogenous neural progenitor/stem cell-mediated regenerative response after neural injury. Increased and sustained bioavailability of SDF-1α in the peri-injury region is hypothesized to modulate this endogenous repair response. Here, we describe poly(lactic-co-glycolic) acid (PLGA) nanoparticles capable of releasing bioactive SDF-1α in a sustained manner over 60 days after a burst of 23%. Moreover, we report a biphasic cellular response to SDF-1α concentrations thus the large initial burst release in an in vivo setting may result in supratherapeutic concentrations of SDF-1α. Specific protein-protein interactions between SDF-1α and fibrin (as well as its monomer, fibrinogen) were exploited to control the magnitude of the burst release. Nanoparticles embedded in fibrin significantly reduced the amount of SDF-1α released after 72 h as a function of fibrin density. Therefore, the nanoparticle/fibrin composites represented a means to independently tune the magnitude of the burst phase release from the nanoparticles while perserving a bioactive depot of SDF-1α for release over 60 days.

Original languageEnglish (US)
Pages (from-to)7963-7973
Number of pages11
JournalJournal of Materials Chemistry B
Volume3
Issue number40
DOIs
StatePublished - 2015

Fingerprint

Chemokine CXCL12
Fibrin
Nanoparticles
Proteins
Composite materials
Stem cells
Repair
Monomers
Neural Stem Cells
Acids
Wounds and Injuries
Chemokines
Fibrinogen
Biological Availability
Stem Cells

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine(all)
  • Chemistry(all)
  • Materials Science(all)

Cite this

Tunable controlled release of bioactive SDF-1α via specific protein interactions within fibrin/nanoparticle composites. / Dutta, D.; Fauer, C.; Mulleneux, H. L.; Stabenfeldt, Sarah.

In: Journal of Materials Chemistry B, Vol. 3, No. 40, 2015, p. 7963-7973.

Research output: Contribution to journalArticle

@article{01906ada24404fe0ae8ce76b96f69a40,
title = "Tunable controlled release of bioactive SDF-1α via specific protein interactions within fibrin/nanoparticle composites",
abstract = "The chemokine, stromal cell-derived factor 1α (SDF-1α), is a key regulator of the endogenous neural progenitor/stem cell-mediated regenerative response after neural injury. Increased and sustained bioavailability of SDF-1α in the peri-injury region is hypothesized to modulate this endogenous repair response. Here, we describe poly(lactic-co-glycolic) acid (PLGA) nanoparticles capable of releasing bioactive SDF-1α in a sustained manner over 60 days after a burst of 23{\%}. Moreover, we report a biphasic cellular response to SDF-1α concentrations thus the large initial burst release in an in vivo setting may result in supratherapeutic concentrations of SDF-1α. Specific protein-protein interactions between SDF-1α and fibrin (as well as its monomer, fibrinogen) were exploited to control the magnitude of the burst release. Nanoparticles embedded in fibrin significantly reduced the amount of SDF-1α released after 72 h as a function of fibrin density. Therefore, the nanoparticle/fibrin composites represented a means to independently tune the magnitude of the burst phase release from the nanoparticles while perserving a bioactive depot of SDF-1α for release over 60 days.",
author = "D. Dutta and C. Fauer and Mulleneux, {H. L.} and Sarah Stabenfeldt",
year = "2015",
doi = "10.1039/c5tb00935a",
language = "English (US)",
volume = "3",
pages = "7963--7973",
journal = "Journal of Materials Chemistry B",
issn = "2050-7518",
publisher = "Royal Society of Chemistry",
number = "40",

}

TY - JOUR

T1 - Tunable controlled release of bioactive SDF-1α via specific protein interactions within fibrin/nanoparticle composites

AU - Dutta, D.

AU - Fauer, C.

AU - Mulleneux, H. L.

AU - Stabenfeldt, Sarah

PY - 2015

Y1 - 2015

N2 - The chemokine, stromal cell-derived factor 1α (SDF-1α), is a key regulator of the endogenous neural progenitor/stem cell-mediated regenerative response after neural injury. Increased and sustained bioavailability of SDF-1α in the peri-injury region is hypothesized to modulate this endogenous repair response. Here, we describe poly(lactic-co-glycolic) acid (PLGA) nanoparticles capable of releasing bioactive SDF-1α in a sustained manner over 60 days after a burst of 23%. Moreover, we report a biphasic cellular response to SDF-1α concentrations thus the large initial burst release in an in vivo setting may result in supratherapeutic concentrations of SDF-1α. Specific protein-protein interactions between SDF-1α and fibrin (as well as its monomer, fibrinogen) were exploited to control the magnitude of the burst release. Nanoparticles embedded in fibrin significantly reduced the amount of SDF-1α released after 72 h as a function of fibrin density. Therefore, the nanoparticle/fibrin composites represented a means to independently tune the magnitude of the burst phase release from the nanoparticles while perserving a bioactive depot of SDF-1α for release over 60 days.

AB - The chemokine, stromal cell-derived factor 1α (SDF-1α), is a key regulator of the endogenous neural progenitor/stem cell-mediated regenerative response after neural injury. Increased and sustained bioavailability of SDF-1α in the peri-injury region is hypothesized to modulate this endogenous repair response. Here, we describe poly(lactic-co-glycolic) acid (PLGA) nanoparticles capable of releasing bioactive SDF-1α in a sustained manner over 60 days after a burst of 23%. Moreover, we report a biphasic cellular response to SDF-1α concentrations thus the large initial burst release in an in vivo setting may result in supratherapeutic concentrations of SDF-1α. Specific protein-protein interactions between SDF-1α and fibrin (as well as its monomer, fibrinogen) were exploited to control the magnitude of the burst release. Nanoparticles embedded in fibrin significantly reduced the amount of SDF-1α released after 72 h as a function of fibrin density. Therefore, the nanoparticle/fibrin composites represented a means to independently tune the magnitude of the burst phase release from the nanoparticles while perserving a bioactive depot of SDF-1α for release over 60 days.

UR - http://www.scopus.com/inward/record.url?scp=84943628041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943628041&partnerID=8YFLogxK

U2 - 10.1039/c5tb00935a

DO - 10.1039/c5tb00935a

M3 - Article

VL - 3

SP - 7963

EP - 7973

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

SN - 2050-7518

IS - 40

ER -