Tumor necrosis factor-α plays a role in host defense against Histoplasma capsulatum

Jeffrey G. Smith, Dewey Magee, Dwight M. Williams, John R. Graybill

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Tumor necrosis factor-α was detected in supernatants collected from EALB/c mouse peritoneal macrophages incubated continuously with Histoplasma capsulatum. The levels of TNFα measured by actinomycin D bioassay peaked within hours after exposure and then greatly declined by 24 h. TNFα was also measured in bronchoalveolar lavage fluid from BALB/c mice challenged intranasally with H. capsulatum. Lavage fluid TNFα levels exhibited the same pattern as the in vitro supernatants; they peaked within hours after challenge and lower levels were detected at 24 h. Treatment of mice with anti-TNFα antibody accelerated mortality in response to systemic infection and significantly increased tissue colony counts in the liver and spleen. In the murine model, TNFα is produced in response to H. capsulatum and appears to play some role in host defense to infection.

Original languageEnglish (US)
Pages (from-to)1349-1353
Number of pages5
JournalJournal of Infectious Diseases
Volume162
Issue number6
StatePublished - Dec 1990
Externally publishedYes

Fingerprint

Histoplasma
Tumor Necrosis Factor-alpha
Therapeutic Irrigation
Bronchoalveolar Lavage Fluid
Peritoneal Macrophages
Dactinomycin
Infection
Biological Assay
Anti-Idiotypic Antibodies
Spleen
Mortality
Liver
Therapeutics

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Tumor necrosis factor-α plays a role in host defense against Histoplasma capsulatum. / Smith, Jeffrey G.; Magee, Dewey; Williams, Dwight M.; Graybill, John R.

In: Journal of Infectious Diseases, Vol. 162, No. 6, 12.1990, p. 1349-1353.

Research output: Contribution to journalArticle

Smith, Jeffrey G. ; Magee, Dewey ; Williams, Dwight M. ; Graybill, John R. / Tumor necrosis factor-α plays a role in host defense against Histoplasma capsulatum. In: Journal of Infectious Diseases. 1990 ; Vol. 162, No. 6. pp. 1349-1353.
@article{46b6f88377474be3abc61eeceb015311,
title = "Tumor necrosis factor-α plays a role in host defense against Histoplasma capsulatum",
abstract = "Tumor necrosis factor-α was detected in supernatants collected from EALB/c mouse peritoneal macrophages incubated continuously with Histoplasma capsulatum. The levels of TNFα measured by actinomycin D bioassay peaked within hours after exposure and then greatly declined by 24 h. TNFα was also measured in bronchoalveolar lavage fluid from BALB/c mice challenged intranasally with H. capsulatum. Lavage fluid TNFα levels exhibited the same pattern as the in vitro supernatants; they peaked within hours after challenge and lower levels were detected at 24 h. Treatment of mice with anti-TNFα antibody accelerated mortality in response to systemic infection and significantly increased tissue colony counts in the liver and spleen. In the murine model, TNFα is produced in response to H. capsulatum and appears to play some role in host defense to infection.",
author = "Smith, {Jeffrey G.} and Dewey Magee and Williams, {Dwight M.} and Graybill, {John R.}",
year = "1990",
month = "12",
language = "English (US)",
volume = "162",
pages = "1349--1353",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Tumor necrosis factor-α plays a role in host defense against Histoplasma capsulatum

AU - Smith, Jeffrey G.

AU - Magee, Dewey

AU - Williams, Dwight M.

AU - Graybill, John R.

PY - 1990/12

Y1 - 1990/12

N2 - Tumor necrosis factor-α was detected in supernatants collected from EALB/c mouse peritoneal macrophages incubated continuously with Histoplasma capsulatum. The levels of TNFα measured by actinomycin D bioassay peaked within hours after exposure and then greatly declined by 24 h. TNFα was also measured in bronchoalveolar lavage fluid from BALB/c mice challenged intranasally with H. capsulatum. Lavage fluid TNFα levels exhibited the same pattern as the in vitro supernatants; they peaked within hours after challenge and lower levels were detected at 24 h. Treatment of mice with anti-TNFα antibody accelerated mortality in response to systemic infection and significantly increased tissue colony counts in the liver and spleen. In the murine model, TNFα is produced in response to H. capsulatum and appears to play some role in host defense to infection.

AB - Tumor necrosis factor-α was detected in supernatants collected from EALB/c mouse peritoneal macrophages incubated continuously with Histoplasma capsulatum. The levels of TNFα measured by actinomycin D bioassay peaked within hours after exposure and then greatly declined by 24 h. TNFα was also measured in bronchoalveolar lavage fluid from BALB/c mice challenged intranasally with H. capsulatum. Lavage fluid TNFα levels exhibited the same pattern as the in vitro supernatants; they peaked within hours after challenge and lower levels were detected at 24 h. Treatment of mice with anti-TNFα antibody accelerated mortality in response to systemic infection and significantly increased tissue colony counts in the liver and spleen. In the murine model, TNFα is produced in response to H. capsulatum and appears to play some role in host defense to infection.

UR - http://www.scopus.com/inward/record.url?scp=0025201559&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025201559&partnerID=8YFLogxK

M3 - Article

C2 - 2230264

AN - SCOPUS:0025201559

VL - 162

SP - 1349

EP - 1353

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 6

ER -