Tumor necrosis factor-α plays a role in host defense against histoplasma capsulatum

Jeffrey G. Smith, D. Mitchell Magee, Dwight M. Williams, John R. Graybill

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

Tumor necrosis factor-α was detected in supernatants collected from BALB/c mouse peritoneal macrophages incubated continuously with Histoplasma capsulatum. The levels of TNFα measured by actinomycin D bioassay peaked within hours after exposure and then greatly declined by 24 h. TNFα was also measured in bronchoalveolar lavage fluid from BALB/cmice challenged intranasally with H. capsulatum. Lavage fluid TNFα levels exhibited the same pattern as the in vitro supernatants; they peaked within hours after challenge and lower levels were detected at 24 h. Treatment of mice with anti-TNFα antibody accelerated mortality in response to systemic infection and significantly increased tissue colony counts in the liver and spleen. In the murine model, TNFα is produced in response to H. capsulatum and appears to play some role in host defense to infection.

Original languageEnglish (US)
Pages (from-to)1349-1353
Number of pages5
JournalJournal of Infectious Diseases
Volume162
Issue number6
DOIs
StatePublished - Dec 1990
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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