Tropism of Tanapox virus infection in primary human cells

Steven H. Nazarian, John W. Barrett, Marianne M. Stanford, James B. Johnston, Karim Essani, Douglas McFadden

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Tanapox virus (TPV) belongs to the genus Yatapoxvirus and causes a relatively benign zoonotic disease in man, with symptoms that resemble a mild version of human monkeypox. In order to investigate the underlying mechanisms of TPV pathogenesis, the tropism and replication characteristics of TPV were examined in a variety of primary human cells. A GFP expressing TPV (TPV-GFP) was constructed and used to infect primary human dermal fibroblasts (pHDFs) and peripheral blood mononuclear cells (PBMCs), both of which are believed to be major in vivo targets of poxvirus infection. pHDFs fully supported productive replication and cell-cell spread of TPV-GFP. However, induction of cell cycle arrest in pHDFs by contact mediated inhibition or rapamycin treatment eliminated the ability of TPV to fully stimulate cell cycle progression and dramatically reduced viral replication. TPV-GFP-infected human PBMCs were screened for permissiveness by FACS analysis. CD14+ cells (monocytes) were the primary cellular target for TPV infection. A small proportion of CD3+ cells (T cells) were positive for GFP expression, yet TPV was not able to replicate and spread in cultured peripheral blood lymphocytes, regardless of their state of activation. Primary human monocytes, however, demonstrated robust TPV replication, yet these cells no longer supported replication of TPV once they differentiated into macrophages. This unique ex vivo tropism of TPV gives key insights into the basis for the self-limiting pathogenicity of TPV in man.

Original languageEnglish (US)
Pages (from-to)32-40
Number of pages9
JournalVirology
Volume368
Issue number1
DOIs
StatePublished - Nov 10 2007
Externally publishedYes

Fingerprint

Yatapoxvirus
Tropism
Virus Diseases
Fibroblasts
Skin
Monocytes
Blood Cells
Monkeypox
Poxviridae Infections

Keywords

  • Pathogenesis
  • Poxvirus
  • Primary human cells
  • Replication
  • Tanapox

ASJC Scopus subject areas

  • Virology

Cite this

Nazarian, S. H., Barrett, J. W., Stanford, M. M., Johnston, J. B., Essani, K., & McFadden, D. (2007). Tropism of Tanapox virus infection in primary human cells. Virology, 368(1), 32-40. https://doi.org/10.1016/j.virol.2007.06.019

Tropism of Tanapox virus infection in primary human cells. / Nazarian, Steven H.; Barrett, John W.; Stanford, Marianne M.; Johnston, James B.; Essani, Karim; McFadden, Douglas.

In: Virology, Vol. 368, No. 1, 10.11.2007, p. 32-40.

Research output: Contribution to journalArticle

Nazarian, SH, Barrett, JW, Stanford, MM, Johnston, JB, Essani, K & McFadden, D 2007, 'Tropism of Tanapox virus infection in primary human cells', Virology, vol. 368, no. 1, pp. 32-40. https://doi.org/10.1016/j.virol.2007.06.019
Nazarian SH, Barrett JW, Stanford MM, Johnston JB, Essani K, McFadden D. Tropism of Tanapox virus infection in primary human cells. Virology. 2007 Nov 10;368(1):32-40. https://doi.org/10.1016/j.virol.2007.06.019
Nazarian, Steven H. ; Barrett, John W. ; Stanford, Marianne M. ; Johnston, James B. ; Essani, Karim ; McFadden, Douglas. / Tropism of Tanapox virus infection in primary human cells. In: Virology. 2007 ; Vol. 368, No. 1. pp. 32-40.
@article{0828b2fbea9d4634bb9f329f98bd44ad,
title = "Tropism of Tanapox virus infection in primary human cells",
abstract = "Tanapox virus (TPV) belongs to the genus Yatapoxvirus and causes a relatively benign zoonotic disease in man, with symptoms that resemble a mild version of human monkeypox. In order to investigate the underlying mechanisms of TPV pathogenesis, the tropism and replication characteristics of TPV were examined in a variety of primary human cells. A GFP expressing TPV (TPV-GFP) was constructed and used to infect primary human dermal fibroblasts (pHDFs) and peripheral blood mononuclear cells (PBMCs), both of which are believed to be major in vivo targets of poxvirus infection. pHDFs fully supported productive replication and cell-cell spread of TPV-GFP. However, induction of cell cycle arrest in pHDFs by contact mediated inhibition or rapamycin treatment eliminated the ability of TPV to fully stimulate cell cycle progression and dramatically reduced viral replication. TPV-GFP-infected human PBMCs were screened for permissiveness by FACS analysis. CD14+ cells (monocytes) were the primary cellular target for TPV infection. A small proportion of CD3+ cells (T cells) were positive for GFP expression, yet TPV was not able to replicate and spread in cultured peripheral blood lymphocytes, regardless of their state of activation. Primary human monocytes, however, demonstrated robust TPV replication, yet these cells no longer supported replication of TPV once they differentiated into macrophages. This unique ex vivo tropism of TPV gives key insights into the basis for the self-limiting pathogenicity of TPV in man.",
keywords = "Pathogenesis, Poxvirus, Primary human cells, Replication, Tanapox",
author = "Nazarian, {Steven H.} and Barrett, {John W.} and Stanford, {Marianne M.} and Johnston, {James B.} and Karim Essani and Douglas McFadden",
year = "2007",
month = "11",
day = "10",
doi = "10.1016/j.virol.2007.06.019",
language = "English (US)",
volume = "368",
pages = "32--40",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Tropism of Tanapox virus infection in primary human cells

AU - Nazarian, Steven H.

AU - Barrett, John W.

AU - Stanford, Marianne M.

AU - Johnston, James B.

AU - Essani, Karim

AU - McFadden, Douglas

PY - 2007/11/10

Y1 - 2007/11/10

N2 - Tanapox virus (TPV) belongs to the genus Yatapoxvirus and causes a relatively benign zoonotic disease in man, with symptoms that resemble a mild version of human monkeypox. In order to investigate the underlying mechanisms of TPV pathogenesis, the tropism and replication characteristics of TPV were examined in a variety of primary human cells. A GFP expressing TPV (TPV-GFP) was constructed and used to infect primary human dermal fibroblasts (pHDFs) and peripheral blood mononuclear cells (PBMCs), both of which are believed to be major in vivo targets of poxvirus infection. pHDFs fully supported productive replication and cell-cell spread of TPV-GFP. However, induction of cell cycle arrest in pHDFs by contact mediated inhibition or rapamycin treatment eliminated the ability of TPV to fully stimulate cell cycle progression and dramatically reduced viral replication. TPV-GFP-infected human PBMCs were screened for permissiveness by FACS analysis. CD14+ cells (monocytes) were the primary cellular target for TPV infection. A small proportion of CD3+ cells (T cells) were positive for GFP expression, yet TPV was not able to replicate and spread in cultured peripheral blood lymphocytes, regardless of their state of activation. Primary human monocytes, however, demonstrated robust TPV replication, yet these cells no longer supported replication of TPV once they differentiated into macrophages. This unique ex vivo tropism of TPV gives key insights into the basis for the self-limiting pathogenicity of TPV in man.

AB - Tanapox virus (TPV) belongs to the genus Yatapoxvirus and causes a relatively benign zoonotic disease in man, with symptoms that resemble a mild version of human monkeypox. In order to investigate the underlying mechanisms of TPV pathogenesis, the tropism and replication characteristics of TPV were examined in a variety of primary human cells. A GFP expressing TPV (TPV-GFP) was constructed and used to infect primary human dermal fibroblasts (pHDFs) and peripheral blood mononuclear cells (PBMCs), both of which are believed to be major in vivo targets of poxvirus infection. pHDFs fully supported productive replication and cell-cell spread of TPV-GFP. However, induction of cell cycle arrest in pHDFs by contact mediated inhibition or rapamycin treatment eliminated the ability of TPV to fully stimulate cell cycle progression and dramatically reduced viral replication. TPV-GFP-infected human PBMCs were screened for permissiveness by FACS analysis. CD14+ cells (monocytes) were the primary cellular target for TPV infection. A small proportion of CD3+ cells (T cells) were positive for GFP expression, yet TPV was not able to replicate and spread in cultured peripheral blood lymphocytes, regardless of their state of activation. Primary human monocytes, however, demonstrated robust TPV replication, yet these cells no longer supported replication of TPV once they differentiated into macrophages. This unique ex vivo tropism of TPV gives key insights into the basis for the self-limiting pathogenicity of TPV in man.

KW - Pathogenesis

KW - Poxvirus

KW - Primary human cells

KW - Replication

KW - Tanapox

UR - http://www.scopus.com/inward/record.url?scp=35348932699&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35348932699&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2007.06.019

DO - 10.1016/j.virol.2007.06.019

M3 - Article

C2 - 17632198

AN - SCOPUS:35348932699

VL - 368

SP - 32

EP - 40

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -