TY - JOUR
T1 - Transient infectivity and heterokaryon formation in hamster cell cultures inoculated with cell-associated stocks of Marek’s disease virus and herpesvirus of turkeys
AU - Witter, R. L.
AU - Sharma, J. M.
PY - 1974/12
Y1 - 1974/12
N2 - Virus infectivity persisted for up to 10 serial passages in primary hamster kidney (HK) cultures inoculated with cell-associated Marek’s disease virus (MDV) or herpesvirus of turkeys (HVT). However, the direct correlation of infectivity with the persistence of at least 106 inoculum cells/culture, the ablation of infectivity by treatment with cytotoxic anti-avian cell antibody, and the failure to infect HK cultures with high doses of cell-free MDV or HVT strongly indicated that infection was solely associated with inoculum-derived avian cells. Heterokaryons containing avian and hamster nuclei as well as viral antigens were observed in HK cultures inoculated with virus-infected avian cells, but the function of these heterokaryons in viral replication was uncertain. It was concluded that HK cells did not support persistent infection with, and were probably refractory to, the strains of MDV and HVT used.
AB - Virus infectivity persisted for up to 10 serial passages in primary hamster kidney (HK) cultures inoculated with cell-associated Marek’s disease virus (MDV) or herpesvirus of turkeys (HVT). However, the direct correlation of infectivity with the persistence of at least 106 inoculum cells/culture, the ablation of infectivity by treatment with cytotoxic anti-avian cell antibody, and the failure to infect HK cultures with high doses of cell-free MDV or HVT strongly indicated that infection was solely associated with inoculum-derived avian cells. Heterokaryons containing avian and hamster nuclei as well as viral antigens were observed in HK cultures inoculated with virus-infected avian cells, but the function of these heterokaryons in viral replication was uncertain. It was concluded that HK cells did not support persistent infection with, and were probably refractory to, the strains of MDV and HVT used.
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U2 - 10.1093/jnci/53.6.1731
DO - 10.1093/jnci/53.6.1731
M3 - Article
C2 - 4612166
AN - SCOPUS:0016332696
SN - 0027-8874
VL - 53
SP - 1731
EP - 1742
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 6
ER -