Transforming growth factor alpha, shope fibroma growth factor, and vaccinia growth factor can replace myxoma growth factor in the induction of myxomatosis in rabbits

Andrea Opgenorth, Nick Nation, Kathryn Graham, Douglas McFadden

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The epidermal growth factor (EGF) homologues encoded by vaccinia virus, myxoma virus, and malignant rabbit fibroma virus have been shown to contribute to the pathogenicity of virus infection upon inoculation of susceptible hosts. However, since the primary structures of these growth factors and the disease profiles induced by different poxvirus genera vary substantially, the degree to which the various EGF homologues perform similar roles in viral pathogenesis remains unclear. In order to determine whether different EGF-like growth factors can perform qualitatively similar functions in the induction of myxomatosis in rabbits, we created recombinant myxoma virus variants in which the native growth factor, myxoma growth factor (MGF), was disrupted and replaced with either vaccinia virus growth factor, Shope fibroma growth factor, or rat transforming growth factor alpha. Unlike the control virus containing an inactivated MGF gene, which caused marked attenuation of the disease syndrome and substantially less proliferation of the epithelial cell layers in the conjunctiva and respiratory tract, the recombinant myxoma virus strains expressing heterologous growth factors produced infections which were both clinically and histopathologically indistinguishable from wild-type myxomatosis. We conclude that these poxviral and cellular EGF-like growth factors, which are diverse with respect to primary structure and origin, have similar biological functions in the context of myxoma virus pathogenesis and are mitogenic for the same target cells.

Original languageEnglish (US)
Pages (from-to)701-709
Number of pages9
JournalVirology
Volume192
Issue number2
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

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Myxoma virus
Transforming Growth Factor alpha
Intercellular Signaling Peptides and Proteins
Epidermal Growth Factor
Rabbits
Rabbit Fibroma Virus
Poxviridae
Vaccinia virus
Conjunctiva
Virus Diseases
Respiratory System
Virulence
Epithelial Cells
myxoma virus growth factor
Shope fibroma virus growth factor
vaccinia growth factor
Viruses
Infection
Genes

ASJC Scopus subject areas

  • Virology

Cite this

Transforming growth factor alpha, shope fibroma growth factor, and vaccinia growth factor can replace myxoma growth factor in the induction of myxomatosis in rabbits. / Opgenorth, Andrea; Nation, Nick; Graham, Kathryn; McFadden, Douglas.

In: Virology, Vol. 192, No. 2, 01.01.1993, p. 701-709.

Research output: Contribution to journalArticle

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