Transforming growth factor alpha gene expression in cultured human keratinocytes is unaffected by cellular aging

Carolyn Compton, Y. Tong, N. Trookman, H. Zhao, D. Roy, W. Press

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Cultured human keratinocyte grafts have been shown to stimulate endogenous reepithelialization of both chronic nonhealing and acute partial-thickness wounds. This effect is most likely mediated by cytokines that stimulate keratinocyte growth, such as transforming growth factor alpha. The effect of cellular age on cytokine expression by cultured grafts used for this purpose is presently undefined. In this study, transforming growth factor alpha gene expression in cultured foreskin keratinocytes from donors varying m age from 2 to 82 years was analyzed semiquantitatively by two separate methods, ie, Northern hybridization and competitive polymerase chain reaction. Results: No pattern of decline in transforming growth factor alpha messenger RNA expression with increasing cellular age was observed by either analysis. Conclusion: The results indicate that expression of transforming growth factor alpha by cultured grafts may not be significantly affected by increasing cellular age and suggest that, even in the elderly, cultured autografts may be effective as pharmacologic agents for wound treatment.

Original languageEnglish (US)
Pages (from-to)683-690
Number of pages8
JournalArchives of Dermatology
Volume131
Issue number6
StatePublished - 1995
Externally publishedYes

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Transforming Growth Factor alpha
Cell Aging
Keratinocytes
Gene Expression
Transplants
Cytokines
Foreskin
Autografts
Wounds and Injuries
Tissue Donors
Polymerase Chain Reaction
Messenger RNA
Growth
Therapeutics

ASJC Scopus subject areas

  • Dermatology

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Transforming growth factor alpha gene expression in cultured human keratinocytes is unaffected by cellular aging. / Compton, Carolyn; Tong, Y.; Trookman, N.; Zhao, H.; Roy, D.; Press, W.

In: Archives of Dermatology, Vol. 131, No. 6, 1995, p. 683-690.

Research output: Contribution to journalArticle

Compton, Carolyn ; Tong, Y. ; Trookman, N. ; Zhao, H. ; Roy, D. ; Press, W. / Transforming growth factor alpha gene expression in cultured human keratinocytes is unaffected by cellular aging. In: Archives of Dermatology. 1995 ; Vol. 131, No. 6. pp. 683-690.
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