TY - JOUR
T1 - Transformation of mono- and dichlorinated phenoxybenzoates by phenoxybenzoate-dioxygenase in Pseudomonas pseudoalcaligenes POB310 and a modified diarylether-metabolizing bacterium
AU - Halden, Rolf U.
AU - Peters, Eric G.
AU - Halden, Barbara G.
AU - Dwyer, Daryl F.
PY - 2000/7/5
Y1 - 2000/7/5
N2 - Pseudomonas pseudoalcaligenes POB310 contains genes that encode phenoxybenzoate dioxygenase. The enzyme transforms mono- and dichlorinated phenoxybenzoates to yield protocatechuate that is used as a growth substrate and chlorophenols that are nonmetabolizable. Mass spectral analysis of 18O metabolites obtained from the protocatechuate 3,4-dioxygenase-deficient mutant, POB310-B1, suggested that the reaction mechanism is a regioselective angular dioxygenation. A cloning vector containing reaction relevant genes (pD30.9) was transferred into Pseudomonas sp. strain B13 containing a modified ortho-cleavage pathway for aromatic compounds. The resultant Pseudomonas sp. strain B13-D5 (pD30.9) completely metabolized 3-(4- chlorophenoxy)benzoate. During growth on 3-phenoxybenzoate, strain B13-D5 (pD30.9) (K(s) = 0.70 ± 0.04 mM, μ(max) = 0.45 ± 0.03 h1, t(d) = 1.5 h, Y = 0.45 ± 0.03 g biomass · g substrate-1) was better adapted to low substrate concentrations, had a faster rate of growth, and a greater yield than POB310 (K(s) = 1.13 ± 0.06 mM, μ(max) = 0.31 ± 0.02 h-1, t(d) = 2.2 h, Y = 0.39 ± 0.02 g biomass · g substrate-1). (C) 2000 John Wiley and Sons, Inc.
AB - Pseudomonas pseudoalcaligenes POB310 contains genes that encode phenoxybenzoate dioxygenase. The enzyme transforms mono- and dichlorinated phenoxybenzoates to yield protocatechuate that is used as a growth substrate and chlorophenols that are nonmetabolizable. Mass spectral analysis of 18O metabolites obtained from the protocatechuate 3,4-dioxygenase-deficient mutant, POB310-B1, suggested that the reaction mechanism is a regioselective angular dioxygenation. A cloning vector containing reaction relevant genes (pD30.9) was transferred into Pseudomonas sp. strain B13 containing a modified ortho-cleavage pathway for aromatic compounds. The resultant Pseudomonas sp. strain B13-D5 (pD30.9) completely metabolized 3-(4- chlorophenoxy)benzoate. During growth on 3-phenoxybenzoate, strain B13-D5 (pD30.9) (K(s) = 0.70 ± 0.04 mM, μ(max) = 0.45 ± 0.03 h1, t(d) = 1.5 h, Y = 0.45 ± 0.03 g biomass · g substrate-1) was better adapted to low substrate concentrations, had a faster rate of growth, and a greater yield than POB310 (K(s) = 1.13 ± 0.06 mM, μ(max) = 0.31 ± 0.02 h-1, t(d) = 2.2 h, Y = 0.39 ± 0.02 g biomass · g substrate-1). (C) 2000 John Wiley and Sons, Inc.
KW - Chlorinated diarylethers
KW - POB-dioxygenase
KW - Pseudomonas
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U2 - 10.1002/(SICI)1097-0290(20000705)69:1<107::AID-BIT13>3.0.CO;2-T
DO - 10.1002/(SICI)1097-0290(20000705)69:1<107::AID-BIT13>3.0.CO;2-T
M3 - Article
C2 - 10820337
AN - SCOPUS:0034609027
VL - 69
SP - 107
EP - 112
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 1
ER -