Transfer of protective immunity in murine histoplasmosis by a CD4+ T- cell clone

R. Allendoerfer, Dewey Magee, G. S. Deepe, J. R. Graybill

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have reported that a murine Histoplasma capsulatum-reactive CD4+ T- cell line and clones thereof did not adoptively transfer protection against H. capsulatum infection in normal or cyclophosphamide-treated C57BL/6 mice. One explanation for the results was that the T cells failed to traffic to lymphoid organs in these animals. In this study, we have sought to determine whether one of these clones, 2.3H3, could mediate protection in nude (C57BL/10) or irradiated (5 Gy) heterozygous nude (nu/+) C57BL/6 mice. Mice were inoculated intravenously with 107 resting 2.3H3 cells or with an equal number of cells of the ovalbumin-reactive clone 1S6; 2 h later, the mice were challenged intranasally with 5 x 106 yeast cells. By day 5 of infection, lungs, livers, and spleens of nude and irradiated nu/+ mice given 2.3H3 contained significantly fewer (P < 0.05) CFU than the same organs from mice inoculated with 1S6. This effect was specific for H. capsulatum, since 2.3H3 did not reduce the number of Coccidioides immitis CFU in lungs, livers, and spleens of irradiated nu/+ mice. By day 10, the amounts of H. capsulatum CFU in lungs, livers, or spleens of nude and irradiated nu/+ mice inoculated with 2.3H3 were smaller than those in 1S6-inoculated mice, but these differences did not reach statistical significance (P > 0.05). The mortality rate of mice inoculated with 2.3H3 and that of mice inoculated with 1S6 were similar. Histopathological examination of tissues from 2.3H3- and 1S6-inoculated mice demonstrated the presence of granulomatous inflammation in organs from both groups. Tissues from 2.3H3-treated mice contained fewer yeasts per high- power field than tissues from 1S6-treated mice. Thus, irradiated or nude mice are permissive for the expression of protective immunity by a CD4+ T-cell clone. Although the protective capacity of T cells in these animals is transient, these animals will be usmf}l for differentiating protective from nonprotective T-cell clones.

Original languageEnglish (US)
Pages (from-to)714-718
Number of pages5
JournalInfection and Immunity
Volume61
Issue number2
StatePublished - 1993
Externally publishedYes

Fingerprint

Histoplasmosis
Immunity
Clone Cells
T-Lymphocytes
Histoplasma
Inbred C57BL Mouse
Yeasts
Animal Structures
Ovalbumin
Infection
Nude Mice
Cyclophosphamide
Spleen
Cell Count
Inflammation
Cell Line
Lung
Mortality
Liver

ASJC Scopus subject areas

  • Immunology

Cite this

Allendoerfer, R., Magee, D., Deepe, G. S., & Graybill, J. R. (1993). Transfer of protective immunity in murine histoplasmosis by a CD4+ T- cell clone. Infection and Immunity, 61(2), 714-718.

Transfer of protective immunity in murine histoplasmosis by a CD4+ T- cell clone. / Allendoerfer, R.; Magee, Dewey; Deepe, G. S.; Graybill, J. R.

In: Infection and Immunity, Vol. 61, No. 2, 1993, p. 714-718.

Research output: Contribution to journalArticle

Allendoerfer, R, Magee, D, Deepe, GS & Graybill, JR 1993, 'Transfer of protective immunity in murine histoplasmosis by a CD4+ T- cell clone', Infection and Immunity, vol. 61, no. 2, pp. 714-718.
Allendoerfer, R. ; Magee, Dewey ; Deepe, G. S. ; Graybill, J. R. / Transfer of protective immunity in murine histoplasmosis by a CD4+ T- cell clone. In: Infection and Immunity. 1993 ; Vol. 61, No. 2. pp. 714-718.
@article{3f13619ae823442cb6af5f114581135b,
title = "Transfer of protective immunity in murine histoplasmosis by a CD4+ T- cell clone",
abstract = "We have reported that a murine Histoplasma capsulatum-reactive CD4+ T- cell line and clones thereof did not adoptively transfer protection against H. capsulatum infection in normal or cyclophosphamide-treated C57BL/6 mice. One explanation for the results was that the T cells failed to traffic to lymphoid organs in these animals. In this study, we have sought to determine whether one of these clones, 2.3H3, could mediate protection in nude (C57BL/10) or irradiated (5 Gy) heterozygous nude (nu/+) C57BL/6 mice. Mice were inoculated intravenously with 107 resting 2.3H3 cells or with an equal number of cells of the ovalbumin-reactive clone 1S6; 2 h later, the mice were challenged intranasally with 5 x 106 yeast cells. By day 5 of infection, lungs, livers, and spleens of nude and irradiated nu/+ mice given 2.3H3 contained significantly fewer (P < 0.05) CFU than the same organs from mice inoculated with 1S6. This effect was specific for H. capsulatum, since 2.3H3 did not reduce the number of Coccidioides immitis CFU in lungs, livers, and spleens of irradiated nu/+ mice. By day 10, the amounts of H. capsulatum CFU in lungs, livers, or spleens of nude and irradiated nu/+ mice inoculated with 2.3H3 were smaller than those in 1S6-inoculated mice, but these differences did not reach statistical significance (P > 0.05). The mortality rate of mice inoculated with 2.3H3 and that of mice inoculated with 1S6 were similar. Histopathological examination of tissues from 2.3H3- and 1S6-inoculated mice demonstrated the presence of granulomatous inflammation in organs from both groups. Tissues from 2.3H3-treated mice contained fewer yeasts per high- power field than tissues from 1S6-treated mice. Thus, irradiated or nude mice are permissive for the expression of protective immunity by a CD4+ T-cell clone. Although the protective capacity of T cells in these animals is transient, these animals will be usmf}l for differentiating protective from nonprotective T-cell clones.",
author = "R. Allendoerfer and Dewey Magee and Deepe, {G. S.} and Graybill, {J. R.}",
year = "1993",
language = "English (US)",
volume = "61",
pages = "714--718",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Transfer of protective immunity in murine histoplasmosis by a CD4+ T- cell clone

AU - Allendoerfer, R.

AU - Magee, Dewey

AU - Deepe, G. S.

AU - Graybill, J. R.

PY - 1993

Y1 - 1993

N2 - We have reported that a murine Histoplasma capsulatum-reactive CD4+ T- cell line and clones thereof did not adoptively transfer protection against H. capsulatum infection in normal or cyclophosphamide-treated C57BL/6 mice. One explanation for the results was that the T cells failed to traffic to lymphoid organs in these animals. In this study, we have sought to determine whether one of these clones, 2.3H3, could mediate protection in nude (C57BL/10) or irradiated (5 Gy) heterozygous nude (nu/+) C57BL/6 mice. Mice were inoculated intravenously with 107 resting 2.3H3 cells or with an equal number of cells of the ovalbumin-reactive clone 1S6; 2 h later, the mice were challenged intranasally with 5 x 106 yeast cells. By day 5 of infection, lungs, livers, and spleens of nude and irradiated nu/+ mice given 2.3H3 contained significantly fewer (P < 0.05) CFU than the same organs from mice inoculated with 1S6. This effect was specific for H. capsulatum, since 2.3H3 did not reduce the number of Coccidioides immitis CFU in lungs, livers, and spleens of irradiated nu/+ mice. By day 10, the amounts of H. capsulatum CFU in lungs, livers, or spleens of nude and irradiated nu/+ mice inoculated with 2.3H3 were smaller than those in 1S6-inoculated mice, but these differences did not reach statistical significance (P > 0.05). The mortality rate of mice inoculated with 2.3H3 and that of mice inoculated with 1S6 were similar. Histopathological examination of tissues from 2.3H3- and 1S6-inoculated mice demonstrated the presence of granulomatous inflammation in organs from both groups. Tissues from 2.3H3-treated mice contained fewer yeasts per high- power field than tissues from 1S6-treated mice. Thus, irradiated or nude mice are permissive for the expression of protective immunity by a CD4+ T-cell clone. Although the protective capacity of T cells in these animals is transient, these animals will be usmf}l for differentiating protective from nonprotective T-cell clones.

AB - We have reported that a murine Histoplasma capsulatum-reactive CD4+ T- cell line and clones thereof did not adoptively transfer protection against H. capsulatum infection in normal or cyclophosphamide-treated C57BL/6 mice. One explanation for the results was that the T cells failed to traffic to lymphoid organs in these animals. In this study, we have sought to determine whether one of these clones, 2.3H3, could mediate protection in nude (C57BL/10) or irradiated (5 Gy) heterozygous nude (nu/+) C57BL/6 mice. Mice were inoculated intravenously with 107 resting 2.3H3 cells or with an equal number of cells of the ovalbumin-reactive clone 1S6; 2 h later, the mice were challenged intranasally with 5 x 106 yeast cells. By day 5 of infection, lungs, livers, and spleens of nude and irradiated nu/+ mice given 2.3H3 contained significantly fewer (P < 0.05) CFU than the same organs from mice inoculated with 1S6. This effect was specific for H. capsulatum, since 2.3H3 did not reduce the number of Coccidioides immitis CFU in lungs, livers, and spleens of irradiated nu/+ mice. By day 10, the amounts of H. capsulatum CFU in lungs, livers, or spleens of nude and irradiated nu/+ mice inoculated with 2.3H3 were smaller than those in 1S6-inoculated mice, but these differences did not reach statistical significance (P > 0.05). The mortality rate of mice inoculated with 2.3H3 and that of mice inoculated with 1S6 were similar. Histopathological examination of tissues from 2.3H3- and 1S6-inoculated mice demonstrated the presence of granulomatous inflammation in organs from both groups. Tissues from 2.3H3-treated mice contained fewer yeasts per high- power field than tissues from 1S6-treated mice. Thus, irradiated or nude mice are permissive for the expression of protective immunity by a CD4+ T-cell clone. Although the protective capacity of T cells in these animals is transient, these animals will be usmf}l for differentiating protective from nonprotective T-cell clones.

UR - http://www.scopus.com/inward/record.url?scp=0027397443&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027397443&partnerID=8YFLogxK

M3 - Article

C2 - 8093695

AN - SCOPUS:0027397443

VL - 61

SP - 714

EP - 718

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 2

ER -