Transcriptional control of the antimicrobial peptide resistance ugtL gene by the Salmonella Phop and slyA regulatory proteins

Yixin Shi, Tammy Latifi, Michael J. Cromie, Eduardo A. Groisman

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The PhoP/PhoQ two-component system is a master regulator that governs the ability of Salmonella to cause a lethal infection in mice, the adaptation to low Mg2+ environments, and resistance to a variety of antimicrobial peptides. We have recently established that the PhoP-activated ugtL gene is required for resistance to the antimicrobial peptides magainin 2 and polymyxin B. Here we report that ugtL transcription requires not only the PhoP protein but also the virulence regulatory protein SlyA. The PhoP protein footprinted two regions of the ugtL promoter, mutation of either one of which was sufficient to abolish ugtL transcription. Although the SlyA protein is a transcriptional activator of the ugtL gene, it footprinted the ugtL promoter at a region located downstream of the transcription start site. The PhoP protein footprinted the slyA promoter, indicating that it controls slyA transcription directly. The slyA mutant was hypersensitive to magainin 2 and polymyxin B, suggesting that the virulence attenuation exhibited by slyA mutants may be caused by hypersensitivity to antimicrobial peptides. We propose that the PhoP and SlyA proteins control ugtL transcription using a feed-forward loop design.

Original languageEnglish (US)
Pages (from-to)38618-38625
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number37
DOIs
StatePublished - Sep 10 2004
Externally publishedYes

Fingerprint

Salmonella
Genes
Transcription
Peptides
Magainins
Polymyxin B
Proteins
Virulence
Transcription Initiation Site
Genetic Promoter Regions
Hypersensitivity
Mutation
Infection

ASJC Scopus subject areas

  • Biochemistry

Cite this

Transcriptional control of the antimicrobial peptide resistance ugtL gene by the Salmonella Phop and slyA regulatory proteins. / Shi, Yixin; Latifi, Tammy; Cromie, Michael J.; Groisman, Eduardo A.

In: Journal of Biological Chemistry, Vol. 279, No. 37, 10.09.2004, p. 38618-38625.

Research output: Contribution to journalArticle

Shi, Yixin ; Latifi, Tammy ; Cromie, Michael J. ; Groisman, Eduardo A. / Transcriptional control of the antimicrobial peptide resistance ugtL gene by the Salmonella Phop and slyA regulatory proteins. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 37. pp. 38618-38625.
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