Transcription of Djun from D. melanogaster is positively regulated by DTF-1, AP-1, and CREB binding sites

G. L. Wang, Elliott S. Goldstein

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Djun is the homolog of the mammalian proto-oncogene jun in D. melanogaster, where it encodes a component of an AP-1-like nuclear DNA binding protein, or transcription factor. Djun, unlike its vertebrate counterparts, contains an intron in its 5′ noncoding region. The expression of Djun in cultured Schneider line 2 cells is controlled by multiple cis-acting elements in its promoter region and the 5′ noncoding region of the transcription unit. A 43-bp 5′ upstream promoter region is necessary for the transcription activity of Djun. Deletion of this fragment decreased transcriptional activity by 67-fold. This region includes a TATA box and a sequence similar to the Drosophila transcription factor 1 (DTF-1) consensus sequence (GCAACAT/CG/C). A large DNase I footprint covering both the DTF-1 binding site and the TATA box was detected in this region when incubated with nuclear extract from Drosophila embryos, suggesting interactions with related transcription factors. This 43-bp sequence alone, containing the DTF-1 binding site and TATA box, however, is not sufficient for transcription activity. An 80-bp sequence including the start of transcription has considerable basal activity. An intragenic region containing an AP-1 site and a CRE site modulates or fine tunes activity of the promoter. Its activity as an enhancer is reduced when moved upstream in either orientation. An extragenic region containing two AP-1 sites similarly affects promoter activity.

Original languageEnglish (US)
Pages (from-to)389-399
Number of pages11
JournalExperimental Cell Research
Issue number1
StatePublished - Sep 1994

ASJC Scopus subject areas

  • Cell Biology


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