TY - JOUR
T1 - Transcription of Djun from D. melanogaster is positively regulated by DTF-1, AP-1, and CREB binding sites
AU - Wang, G. L.
AU - Goldstein, Elliott S.
PY - 1994/9
Y1 - 1994/9
N2 - Djun is the homolog of the mammalian proto-oncogene jun in D. melanogaster, where it encodes a component of an AP-1-like nuclear DNA binding protein, or transcription factor. Djun, unlike its vertebrate counterparts, contains an intron in its 5′ noncoding region. The expression of Djun in cultured Schneider line 2 cells is controlled by multiple cis-acting elements in its promoter region and the 5′ noncoding region of the transcription unit. A 43-bp 5′ upstream promoter region is necessary for the transcription activity of Djun. Deletion of this fragment decreased transcriptional activity by 67-fold. This region includes a TATA box and a sequence similar to the Drosophila transcription factor 1 (DTF-1) consensus sequence (GCAACAT/CG/C). A large DNase I footprint covering both the DTF-1 binding site and the TATA box was detected in this region when incubated with nuclear extract from Drosophila embryos, suggesting interactions with related transcription factors. This 43-bp sequence alone, containing the DTF-1 binding site and TATA box, however, is not sufficient for transcription activity. An 80-bp sequence including the start of transcription has considerable basal activity. An intragenic region containing an AP-1 site and a CRE site modulates or fine tunes activity of the promoter. Its activity as an enhancer is reduced when moved upstream in either orientation. An extragenic region containing two AP-1 sites similarly affects promoter activity.
AB - Djun is the homolog of the mammalian proto-oncogene jun in D. melanogaster, where it encodes a component of an AP-1-like nuclear DNA binding protein, or transcription factor. Djun, unlike its vertebrate counterparts, contains an intron in its 5′ noncoding region. The expression of Djun in cultured Schneider line 2 cells is controlled by multiple cis-acting elements in its promoter region and the 5′ noncoding region of the transcription unit. A 43-bp 5′ upstream promoter region is necessary for the transcription activity of Djun. Deletion of this fragment decreased transcriptional activity by 67-fold. This region includes a TATA box and a sequence similar to the Drosophila transcription factor 1 (DTF-1) consensus sequence (GCAACAT/CG/C). A large DNase I footprint covering both the DTF-1 binding site and the TATA box was detected in this region when incubated with nuclear extract from Drosophila embryos, suggesting interactions with related transcription factors. This 43-bp sequence alone, containing the DTF-1 binding site and TATA box, however, is not sufficient for transcription activity. An 80-bp sequence including the start of transcription has considerable basal activity. An intragenic region containing an AP-1 site and a CRE site modulates or fine tunes activity of the promoter. Its activity as an enhancer is reduced when moved upstream in either orientation. An extragenic region containing two AP-1 sites similarly affects promoter activity.
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U2 - 10.1006/excr.1994.1272
DO - 10.1006/excr.1994.1272
M3 - Article
C2 - 8082742
AN - SCOPUS:0027964501
SN - 0014-4827
VL - 214
SP - 389
EP - 399
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -