Tracking origins and spread of sulfadoxine-resistant Plasmodium falciparum dhps alleles in Thailand

Md Tauqeer Alam, Sumiti Vinayak, Kanungnit Congpuong, Chansuda Wongsrichanalai, Wichai Satimai, Laurence Slutsker, Ananias A. Escalante, John W. Barnwell, Venkatachalam Udhayakumar

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Abstract

The emergence and spread of drug-resistant Plasmodium falciparum have been a major impediment for the control of malaria worldwide. Earlier studies have shown that similar to chloroquine (CQ) resistance, high levels of pyrimethamine resistance in P. falciparum originated independently 4 to 5 times globally, including one origin at the Thailand-Cambodia border. In this study we describe the origins and spread of sulfadoxineresistance-conferring dihydropteroate synthase (dhps) alleles in Thailand. The dhps mutations and flanking microsatellite loci were genotyped for P. falciparum isolates collected from 11 Thai provinces along the Burma, Cambodia, and Malaysia borders. Results indicated that resistant dhps alleles were fixed in Thailand, predominantly being the SGEGA, AGEAA, and SGNGA triple mutants and the AGKAA double mutant (mutated codons are underlined). These alleles had different geographical distributions. The SGEGA alleles were found mostly at the Burma border, while the SGNGA alleles occurred mainly at the Cambodia border and nearby provinces. Microsatellite data suggested that there were two major genetic lineages of the triple mutants in Thailand, one common for SGEGA/SGNGA alleles and another one independent for AGEAA. Importantly, the newly reported SGNGA alleles possibly originated at the Thailand-Cambodia border. All parasites in the Yala province (Malaysia border) had AGKAA alleles with almost identical flanking microsatellites haplotypes. They were also identical at putatively neutral loci on chromosomes 2 and 3, suggesting a clonal nature of the parasite population in Yala. In summary, this study suggests multiple and independent origins of resistant dhps alleles in Thailand.

Original languageEnglish (US)
Pages (from-to)155-164
Number of pages10
JournalAntimicrobial Agents and Chemotherapy
Volume55
Issue number1
DOIs
StatePublished - Jan 2011

Fingerprint

Sulfadoxine
Thailand
Plasmodium falciparum
Alleles
Cambodia
Microsatellite Repeats
Myanmar
Malaysia
Parasites
Dihydropteroate Synthase
Pyrimethamine
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 2
Chloroquine
Codon
Haplotypes
Malaria

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Alam, M. T., Vinayak, S., Congpuong, K., Wongsrichanalai, C., Satimai, W., Slutsker, L., ... Udhayakumar, V. (2011). Tracking origins and spread of sulfadoxine-resistant Plasmodium falciparum dhps alleles in Thailand. Antimicrobial Agents and Chemotherapy, 55(1), 155-164. https://doi.org/10.1128/AAC.00691-10

Tracking origins and spread of sulfadoxine-resistant Plasmodium falciparum dhps alleles in Thailand. / Alam, Md Tauqeer; Vinayak, Sumiti; Congpuong, Kanungnit; Wongsrichanalai, Chansuda; Satimai, Wichai; Slutsker, Laurence; Escalante, Ananias A.; Barnwell, John W.; Udhayakumar, Venkatachalam.

In: Antimicrobial Agents and Chemotherapy, Vol. 55, No. 1, 01.2011, p. 155-164.

Research output: Contribution to journalArticle

Alam, MT, Vinayak, S, Congpuong, K, Wongsrichanalai, C, Satimai, W, Slutsker, L, Escalante, AA, Barnwell, JW & Udhayakumar, V 2011, 'Tracking origins and spread of sulfadoxine-resistant Plasmodium falciparum dhps alleles in Thailand', Antimicrobial Agents and Chemotherapy, vol. 55, no. 1, pp. 155-164. https://doi.org/10.1128/AAC.00691-10
Alam, Md Tauqeer ; Vinayak, Sumiti ; Congpuong, Kanungnit ; Wongsrichanalai, Chansuda ; Satimai, Wichai ; Slutsker, Laurence ; Escalante, Ananias A. ; Barnwell, John W. ; Udhayakumar, Venkatachalam. / Tracking origins and spread of sulfadoxine-resistant Plasmodium falciparum dhps alleles in Thailand. In: Antimicrobial Agents and Chemotherapy. 2011 ; Vol. 55, No. 1. pp. 155-164.
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