Tracking metabolism and imaging transport in arbuscular mycorrhizal fungi

Berta Bago, Philip E. Pfeffer, Warren Zipfel, Peter Lammers, Yair Shachar-Hill

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

In the last few years the application of modern techniques to the study of arbuscular mycorrhizas has greatly increased our understanding of the mechanisms underlying carbon metabolism in these mutualistic symbioses. Arbuscular mycorrhizal (AM) monoxenic cultures, nuclear magnetic resonance spectroscopy together with isotopic labeling, and analyses of expressed sequence tags (ESTs) have shed light on the metabolic processes taking place in these interactions, particularly in the case of the mycobiont. More recently, in vivo multiphoton microscopy has provided us with some new insights in the allocation and translocation processes which play crucial roles in the distribution of host plant-derived C throughout the fungal colony. In this mini-review we highlight recent advances in these fields, with special attention to the visualization of oleosomes (i.e., lipid bodies) as they move along the long, coenocytic AM fungal hyphae. Volumetric measurements of such oleosomes have allowed us to estimate the flux of triacylglycerides from the intraradical to the extraradical phase of the AM fungal colony. We raise questions and postulate regulatory mechanisms for C metabolism and translocation within the arbuscular mycorrhizal fungal colony.

Original languageEnglish (US)
Pages (from-to)189-197
Number of pages9
JournalPlant and Soil
Volume244
Issue number1-2
DOIs
StatePublished - Jul 2002
Externally publishedYes

Fingerprint

lipid bodies
mycorrhizal fungi
metabolism
fungus
image analysis
translocation
monoxenic culture
vesicular arbuscular mycorrhizae
symbiosis
expressed sequence tags
hyphae
host plant
visualization
nuclear magnetic resonance
nuclear magnetic resonance spectroscopy
microscopy
host plants
lipid
spectroscopy
carbon

Keywords

  • Arbuscular mycorrhizal fungi
  • Carbon metabolism
  • Monoxenic cultures
  • Multiphoton microscopy
  • Nuclear magnetic resonance spectroscopy
  • Nutrient translocation

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

Cite this

Tracking metabolism and imaging transport in arbuscular mycorrhizal fungi. / Bago, Berta; Pfeffer, Philip E.; Zipfel, Warren; Lammers, Peter; Shachar-Hill, Yair.

In: Plant and Soil, Vol. 244, No. 1-2, 07.2002, p. 189-197.

Research output: Contribution to journalArticle

Bago, B, Pfeffer, PE, Zipfel, W, Lammers, P & Shachar-Hill, Y 2002, 'Tracking metabolism and imaging transport in arbuscular mycorrhizal fungi', Plant and Soil, vol. 244, no. 1-2, pp. 189-197. https://doi.org/10.1023/A:1020212328955
Bago, Berta ; Pfeffer, Philip E. ; Zipfel, Warren ; Lammers, Peter ; Shachar-Hill, Yair. / Tracking metabolism and imaging transport in arbuscular mycorrhizal fungi. In: Plant and Soil. 2002 ; Vol. 244, No. 1-2. pp. 189-197.
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