@inproceedings{02937496ef784a4589642fe0a78d68e0,
title = "Towards single cell fingerprinting in microfluidic device format: Single cell manipulation, protein separation and detection",
abstract = "Our single cell analytical experiments for future protein fingerprinting combines microfluidic on-chip protein electrophoresis with latest laser technology for single cell manipulation and (label-free) protein detection, and is presented by the following issues: 1) Single cells were trapped and navigated by optical tweezers (OT) in a PDMS microfluidic device, and lysed at a predefined position. 2) Separation and detection of proteins was achieved with laser induced fluorescence (LIF) detection in the visible (488 nm) as well as in the UV (266 nm) spectral range for label-free protein detection. 3) First successful experiments with single GFP-transfected Sf9 insect cells exhibit distinct single component electropherograms.",
keywords = "Label-free detection, Microfluidics, Protein separation, Single cell analysis",
author = "W. Hellmich and K. Leffhalm and A. Sischka and T. Duong and N. Jensen and K. Niehaus and K. T{\"o}nsing and A. Ros and D. Anselmetti",
year = "2005",
language = "English (US)",
isbn = "0974361119",
series = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Transducer Research Foundation",
pages = "406--408",
booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference",
note = "9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 ; Conference date: 09-10-2005 Through 13-10-2005",
}