Towards single cell fingerprinting in microfluidic device format: Single cell manipulation, protein separation and detection

W. Hellmich, K. Leffhalm, A. Sischka, T. Duong, N. Jensen, K. Niehaus, K. Tönsing, A. Ros, D. Anselmetti

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Our single cell analytical experiments for future protein fingerprinting combines microfluidic on-chip protein electrophoresis with latest laser technology for single cell manipulation and (label-free) protein detection, and is presented by the following issues: 1) Single cells were trapped and navigated by optical tweezers (OT) in a PDMS microfluidic device, and lysed at a predefined position. 2) Separation and detection of proteins was achieved with laser induced fluorescence (LIF) detection in the visible (488 nm) as well as in the UV (266 nm) spectral range for label-free protein detection. 3) First successful experiments with single GFP-transfected Sf9 insect cells exhibit distinct single component electropherograms.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages406-408
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - Jan 1 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

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Keywords

  • Label-free detection
  • Microfluidics
  • Protein separation
  • Single cell analysis

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Hellmich, W., Leffhalm, K., Sischka, A., Duong, T., Jensen, N., Niehaus, K., Tönsing, K., Ros, A., & Anselmetti, D. (2005). Towards single cell fingerprinting in microfluidic device format: Single cell manipulation, protein separation and detection. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 406-408). (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1). Transducer Research Foundation.