Towards a nanoparticle-based prophylactic for maternal autoantibody-related autism

Amir Bolandparvaz, Rian Harriman, Kenneth Alvarez, Kristina Lilova, Zexi Zang, Andy Lam, Elizabeth Edmiston, Alexandra Navrotsky, Natalia Vapniarsky, Judy Van De Water, Jamal S. Lewis

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Recently, the causative agents of Maternal Autoantibody-Related (MAR) autism, pathological autoantibodies and their epitopic targets (e.g. lactate dehydrogenase B [LDH B] peptide), have been identified. Herein, we report on the development of Systems for Nanoparticle-based Autoantibody Reception and Entrapment (SNAREs), which we hypothesized could scavenge disease-propagating MAR autoantibodies from the maternal blood. To demonstrate this functionality, we synthesized 15 nm dextran iron oxide nanoparticles surface-modified with citric acid, methoxy PEG(10 kDa) amine, and LDH B peptide (33.8 μg peptide/cm2). In vitro, we demonstrated significantly lower macrophage uptake for SNAREs compared to control NPs. The hallmark result of this study was the efficacy of the SNAREs to remove 90% of LDH B autoantibody from patient-derived serum. Further, in vitro cytotoxicity testing and a maximal tolerated dose study in mice demonstrated the safety of the SNARE formulation. This work establishes the feasibility of SNAREs as the first-ever prophylactic against MAR autism.

Original languageEnglish (US)
Article number102067
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume21
DOIs
StatePublished - Oct 2019
Externally publishedYes

Keywords

  • Iron oxide
  • Maternal autoantibody-related autism
  • Peptide-functionalized
  • nanoparticles

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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