Toward cell-targeting gene therapy vectors: Selection of cell-binding peptides from random peptide-presenting phage libraries

Michael A. Barry, William J. Dower, Stephen Albert Johnston

Research output: Contribution to journalArticle

304 Scopus citations

Abstract

Ideal gene therapy vectors would be delivered intravenously to transfect only specific cells. Existing vectors only transfect cells in vivo in a manner determined by blood flow and the site of introduction. As a general and systematic approach for generating cell targeting ligands for gene therapy vectors, we have used peptide-presenting phage libraries to select peptides that bind and enter several different cell types. Because of their small size, cell-binding peptides such as these could be incorporated into biological or physical gene therapy vectors. In addition, peptide-presenting phage themselves may also be candidates for gene therapy vectors.

Original languageEnglish (US)
Pages (from-to)299-305
Number of pages7
JournalNature Medicine
Volume2
Issue number3
DOIs
StatePublished - Mar 15 1996
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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