Tomato is a highly effective vehicle for expression and oral immunization with Norwalk virus capsid protein

Xiuren Zhang, Norene A. Buehner, Anne M. Hutson, Mary K. Estes, Hugh Mason

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Norwalk virus (NV) is an important agent of epidemic gastroenteritis, and an oral subunit vaccine shows potential for protection. Recombinant Norwalk virus (rNV) capsid protein expressed in plants assembles virus-like particles (VLPs) that are orally immunogenic in mice and humans. In this article we examine rNV expression in tomato and potato using a plant-optimized gene, and test the immunogenicity of dried tomato fruit and potato tuber fed to mice. The synthetic gene increased rNV expression fourfold in tomato and potato plants, which assembled VLP. Four doses of 0.4 g freeze-dried tomato fruit containing 64 μg rNV (40 μg VLPs) induced NV-specific serum IgG and mucosal IgA in ≥ 80% of mice, while doses of 0.8 g elicited systemic and mucosal antibody responses in all mice. Feedings of 1 g freeze-dried potato tuber containing 120 μg rNV (90 μg VLPs) were required to produce 100% responsiveness. Oxidation of phenolic compounds upon rehydration of dried tuber caused significant VLP instability, thus decreasing immunogenicity. Air-dried tomato fruit stimulated stronger immune responses than freeze-dried fruit of the same mass, perhaps by limiting the destruction of plant cell matrix and membrane systems that occurs with freeze-drying. Thus, rNV in dried transgenic tomato fruit was a more potent immunogen than that in dried potato tubers, based on the total VLPs ingested. These findings support the use of stabilized, dried tomato fruit for oral delivery of subunit vaccines.

Original languageEnglish (US)
Pages (from-to)419-432
Number of pages14
JournalPlant Biotechnology Journal
Volume4
Issue number4
DOIs
StatePublished - Jul 2006

Fingerprint

Norwalk virus
oral vaccination
Capsid Proteins
Lycopersicon esculentum
coat proteins
virus-like particles
Immunization
Virion
Solanum tuberosum
Fruit
tomatoes
potatoes
tubers
fruits
Subunit Vaccines
subunit vaccines
mice
immune response
mouth
Synthetic Genes

Keywords

  • Capsid protein
  • Norwalk virus
  • Recombinant vaccine
  • Transgenic tomato
  • Virus-like particle

ASJC Scopus subject areas

  • Plant Science

Cite this

Tomato is a highly effective vehicle for expression and oral immunization with Norwalk virus capsid protein. / Zhang, Xiuren; Buehner, Norene A.; Hutson, Anne M.; Estes, Mary K.; Mason, Hugh.

In: Plant Biotechnology Journal, Vol. 4, No. 4, 07.2006, p. 419-432.

Research output: Contribution to journalArticle

Zhang, Xiuren ; Buehner, Norene A. ; Hutson, Anne M. ; Estes, Mary K. ; Mason, Hugh. / Tomato is a highly effective vehicle for expression and oral immunization with Norwalk virus capsid protein. In: Plant Biotechnology Journal. 2006 ; Vol. 4, No. 4. pp. 419-432.
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