Tissue expression of transforming growth factor-β1 and transforming growth factor-α during wound healing in human skin explants

Gunnar Kratz, Carolyn Compton

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the dynamic and complex process of wound healing, locally produced growth factors are important mediators, although their actual roles have not been fully established. In the present study, the presence of transforming growth factor-β1 and -α during the re-epithelialization of full-thickness wounds was investigated in an in vitro model of wound healing in human skin. The amounts of transforming growth factor-β1 and -α secreted from the wound area were measured with enzyme immunoassays, and immunohistochemistry was used to study the localization of these two growth factors in the healing wound. The wounds were followed until they were completely re-epithelialized. The results showed a continuous increase in secreted transforming growth factor-β1 throughout the re-epithelialization phase of healing followed by a decrease after its completion. The keratinocytes migrating out from the wound edges showed intense staining for transforming growth factor-β1 which declined to the level of the surrounding epidermis after the wound was covered by a new epidermis. After the skin was wounded, a decrease both in secreted transforming growth factor-α and in immunostaining for this growth factor was apparent. Even though a minor increase in the immunoreactivity for transforming growth factor-α occurred after the completion of re-epithelialization, no increase in secreted transforming growth factor-α could be detected by enzyme immunoassay. These data suggest that keratinocytes modulate their expression of transforming growth factor-β1 and -α during the wound healing process in human skin and that these changes may be controlled in part by autocrine pathways.

Original languageEnglish (US)
Pages (from-to)222-228
Number of pages7
JournalWound Repair and Regeneration
Volume5
Issue number3
StatePublished - Jul 1997
Externally publishedYes

Fingerprint

Transforming Growth Factors
Wound Healing
Skin
Re-Epithelialization
Wounds and Injuries
Intercellular Signaling Peptides and Proteins
Immunoenzyme Techniques
Keratinocytes
Epidermis
Immunohistochemistry
Staining and Labeling

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

@article{8b8981cff7ab46c1913ede0448ad99c7,
title = "Tissue expression of transforming growth factor-β1 and transforming growth factor-α during wound healing in human skin explants",
abstract = "In the dynamic and complex process of wound healing, locally produced growth factors are important mediators, although their actual roles have not been fully established. In the present study, the presence of transforming growth factor-β1 and -α during the re-epithelialization of full-thickness wounds was investigated in an in vitro model of wound healing in human skin. The amounts of transforming growth factor-β1 and -α secreted from the wound area were measured with enzyme immunoassays, and immunohistochemistry was used to study the localization of these two growth factors in the healing wound. The wounds were followed until they were completely re-epithelialized. The results showed a continuous increase in secreted transforming growth factor-β1 throughout the re-epithelialization phase of healing followed by a decrease after its completion. The keratinocytes migrating out from the wound edges showed intense staining for transforming growth factor-β1 which declined to the level of the surrounding epidermis after the wound was covered by a new epidermis. After the skin was wounded, a decrease both in secreted transforming growth factor-α and in immunostaining for this growth factor was apparent. Even though a minor increase in the immunoreactivity for transforming growth factor-α occurred after the completion of re-epithelialization, no increase in secreted transforming growth factor-α could be detected by enzyme immunoassay. These data suggest that keratinocytes modulate their expression of transforming growth factor-β1 and -α during the wound healing process in human skin and that these changes may be controlled in part by autocrine pathways.",
author = "Gunnar Kratz and Carolyn Compton",
year = "1997",
month = "7",
language = "English (US)",
volume = "5",
pages = "222--228",
journal = "Wound Repair and Regeneration",
issn = "1067-1927",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Tissue expression of transforming growth factor-β1 and transforming growth factor-α during wound healing in human skin explants

AU - Kratz, Gunnar

AU - Compton, Carolyn

PY - 1997/7

Y1 - 1997/7

N2 - In the dynamic and complex process of wound healing, locally produced growth factors are important mediators, although their actual roles have not been fully established. In the present study, the presence of transforming growth factor-β1 and -α during the re-epithelialization of full-thickness wounds was investigated in an in vitro model of wound healing in human skin. The amounts of transforming growth factor-β1 and -α secreted from the wound area were measured with enzyme immunoassays, and immunohistochemistry was used to study the localization of these two growth factors in the healing wound. The wounds were followed until they were completely re-epithelialized. The results showed a continuous increase in secreted transforming growth factor-β1 throughout the re-epithelialization phase of healing followed by a decrease after its completion. The keratinocytes migrating out from the wound edges showed intense staining for transforming growth factor-β1 which declined to the level of the surrounding epidermis after the wound was covered by a new epidermis. After the skin was wounded, a decrease both in secreted transforming growth factor-α and in immunostaining for this growth factor was apparent. Even though a minor increase in the immunoreactivity for transforming growth factor-α occurred after the completion of re-epithelialization, no increase in secreted transforming growth factor-α could be detected by enzyme immunoassay. These data suggest that keratinocytes modulate their expression of transforming growth factor-β1 and -α during the wound healing process in human skin and that these changes may be controlled in part by autocrine pathways.

AB - In the dynamic and complex process of wound healing, locally produced growth factors are important mediators, although their actual roles have not been fully established. In the present study, the presence of transforming growth factor-β1 and -α during the re-epithelialization of full-thickness wounds was investigated in an in vitro model of wound healing in human skin. The amounts of transforming growth factor-β1 and -α secreted from the wound area were measured with enzyme immunoassays, and immunohistochemistry was used to study the localization of these two growth factors in the healing wound. The wounds were followed until they were completely re-epithelialized. The results showed a continuous increase in secreted transforming growth factor-β1 throughout the re-epithelialization phase of healing followed by a decrease after its completion. The keratinocytes migrating out from the wound edges showed intense staining for transforming growth factor-β1 which declined to the level of the surrounding epidermis after the wound was covered by a new epidermis. After the skin was wounded, a decrease both in secreted transforming growth factor-α and in immunostaining for this growth factor was apparent. Even though a minor increase in the immunoreactivity for transforming growth factor-α occurred after the completion of re-epithelialization, no increase in secreted transforming growth factor-α could be detected by enzyme immunoassay. These data suggest that keratinocytes modulate their expression of transforming growth factor-β1 and -α during the wound healing process in human skin and that these changes may be controlled in part by autocrine pathways.

UR - http://www.scopus.com/inward/record.url?scp=0008023048&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0008023048&partnerID=8YFLogxK

M3 - Article

VL - 5

SP - 222

EP - 228

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

SN - 1067-1927

IS - 3

ER -