Tissue dissociation with proteolytic enzymes. Adsorption and activity of enzymes at the cell surface

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Proteolytic enzymes used to dissociate kidney tissue have been shown to adsorb to cell surfaces and persist in an active form for as long as tweny-four hours afterwards. The adsorption and activity of pronase and trypsin at the surfaces of freshly dispersed dog kidney cells was demonstrated by ellipsometry. The effect of various cell washing procedures on desorption of the enzymes and the inhibitory effects of serum on enzyme activity were investigated. Proteolytic enzymes adsorbed to the cell surface were found to prevent the formation of the glycoprotein cell coat material at the surface and so interfere with the attachment, spreading and growth of cells on glass. The possible mechanisms underlying the cell injury and death accompanying enzyme adsorption to the cell surface were discussed.

Original languageEnglish (US)
Pages (from-to)359-367
Number of pages9
JournalExperimental Cell Research
Volume65
Issue number2
DOIs
StatePublished - 1971
Externally publishedYes

Fingerprint

Adsorption
Peptide Hydrolases
Enzymes
Kidney
Glycocalyx
Pronase
Trypsin
Glass
Glycoproteins
Cell Death
Dogs
Wounds and Injuries
Growth
Serum

ASJC Scopus subject areas

  • Cell Biology

Cite this

Tissue dissociation with proteolytic enzymes. Adsorption and activity of enzymes at the cell surface. / Poste, George.

In: Experimental Cell Research, Vol. 65, No. 2, 1971, p. 359-367.

Research output: Contribution to journalArticle

@article{efd77128e4684d99ad3e4c04f42f3be7,
title = "Tissue dissociation with proteolytic enzymes. Adsorption and activity of enzymes at the cell surface",
abstract = "Proteolytic enzymes used to dissociate kidney tissue have been shown to adsorb to cell surfaces and persist in an active form for as long as tweny-four hours afterwards. The adsorption and activity of pronase and trypsin at the surfaces of freshly dispersed dog kidney cells was demonstrated by ellipsometry. The effect of various cell washing procedures on desorption of the enzymes and the inhibitory effects of serum on enzyme activity were investigated. Proteolytic enzymes adsorbed to the cell surface were found to prevent the formation of the glycoprotein cell coat material at the surface and so interfere with the attachment, spreading and growth of cells on glass. The possible mechanisms underlying the cell injury and death accompanying enzyme adsorption to the cell surface were discussed.",
author = "George Poste",
year = "1971",
doi = "10.1016/0014-4827(71)90014-0",
language = "English (US)",
volume = "65",
pages = "359--367",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Tissue dissociation with proteolytic enzymes. Adsorption and activity of enzymes at the cell surface

AU - Poste, George

PY - 1971

Y1 - 1971

N2 - Proteolytic enzymes used to dissociate kidney tissue have been shown to adsorb to cell surfaces and persist in an active form for as long as tweny-four hours afterwards. The adsorption and activity of pronase and trypsin at the surfaces of freshly dispersed dog kidney cells was demonstrated by ellipsometry. The effect of various cell washing procedures on desorption of the enzymes and the inhibitory effects of serum on enzyme activity were investigated. Proteolytic enzymes adsorbed to the cell surface were found to prevent the formation of the glycoprotein cell coat material at the surface and so interfere with the attachment, spreading and growth of cells on glass. The possible mechanisms underlying the cell injury and death accompanying enzyme adsorption to the cell surface were discussed.

AB - Proteolytic enzymes used to dissociate kidney tissue have been shown to adsorb to cell surfaces and persist in an active form for as long as tweny-four hours afterwards. The adsorption and activity of pronase and trypsin at the surfaces of freshly dispersed dog kidney cells was demonstrated by ellipsometry. The effect of various cell washing procedures on desorption of the enzymes and the inhibitory effects of serum on enzyme activity were investigated. Proteolytic enzymes adsorbed to the cell surface were found to prevent the formation of the glycoprotein cell coat material at the surface and so interfere with the attachment, spreading and growth of cells on glass. The possible mechanisms underlying the cell injury and death accompanying enzyme adsorption to the cell surface were discussed.

UR - http://www.scopus.com/inward/record.url?scp=0015042480&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015042480&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(71)90014-0

DO - 10.1016/0014-4827(71)90014-0

M3 - Article

VL - 65

SP - 359

EP - 367

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 2

ER -