TIME‐RESOLVED FLUORESCENCE OF NITROBENZOXADIAZOLE‐AMINOHEXANOIC ACID: EFFECT OF INTERMOLECULAR HYDROGEN‐BONDING ON NON‐RADIATIVE DECAY

Su Lin, Walter S. Struve

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

The fluorescence kinetics of the nitrobenzoxadiazole (NBD) chromophore were studied at low concentrations in solvents with varying polarity and hydrogen‐bonding donor strength. The emission decay was essentially single exponential in all solvents studied. While the absorption and fluorescence solvatochromism is determined largely by the solvent polarity, the S, state decay kinetics are strongly modulated by the solvent H‐bonding capacity. The NBD emission lifetime, generally –7–10ns in the aprotic solvents, is reduced to 0.933 ns in water. The solvent deuterium isotope effect on the fluorescence decay is substantial in D20 and in methanol‐d4, but is insignificant in DMSO‐d6. These results are consistent with acceleration of S1S11 internal conversion through an accepting vibrational mode created by intermolecular hydrogen‐bonding of the NBD chromophore to an H atom‐donating solvent. This work bears on the practicality of using NBD as a fluorophore in assays for estrogen and progesterone receptors.

Original languageEnglish (US)
Pages (from-to)361-365
Number of pages5
JournalPhotochemistry and photobiology
Volume54
Issue number3
DOIs
StatePublished - Sep 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

Fingerprint Dive into the research topics of 'TIME‐RESOLVED FLUORESCENCE OF NITROBENZOXADIAZOLE‐AMINOHEXANOIC ACID: EFFECT OF INTERMOLECULAR HYDROGEN‐BONDING ON NON‐RADIATIVE DECAY'. Together they form a unique fingerprint.

  • Cite this