Time-resolved serial femtosecond crystallography at the European XFEL

Suraj Pandey; Richard Bean; Tokushi Sato; Ishwor Poudyal; Johan Bielecki; Jorvani Cruz Villarreal; Oleksandr Yefanov; Valerio Mariani; Thomas A. White; Christopher Kupitz; Mark Hunter; Mohamed H. Abdellatif; Saša Bajt; Valerii Bondar; Austin Echelmeier; Diandra Doppler; Moritz Emons; Matthias Frank; Raimund Fromme; Yaroslav Gevorkov; Gabriele Giovanetti; Man Jiang; Daihyun Kim; Yoonhee Kim; Henry Kirkwood; Anna Klimovskaia; Juraj Knoska; Faisal H.M. Koua; Romain Letrun; Stella Lisova; Luis Maia; Victoria Mazalova; Domingo Meza; Thomas Michelat; Abbas Ourmazd; Guido Palmer; Marco Ramilli; Robin Schubert; Peter Schwander; Alessandro Silenzi; Jolanta Sztuk-Dambietz; Alexandra Tolstikova; Henry N. Chapman; Alexandra Ros; Anton Barty; Petra Fromme; Adrian P. Mancuso; Marius Schmidt

doi:10.1038/s41592-019-0628-z

Time-resolved serial femtosecond crystallography at the European XFEL

Suraj Pandey, Richard Bean, Tokushi Sato, Ishwor Poudyal, Johan Bielecki, Jorvani Cruz Villarreal, Oleksandr Yefanov, Valerio Mariani, Thomas A. White, Christopher Kupitz, Mark Hunter, Mohamed H. Abdellatif, Saša Bajt, Valerii Bondar, Austin Echelmeier, Diandra Doppler, Moritz Emons, Matthias Frank, Raimund Fromme, Yaroslav GevorkovGabriele Giovanetti, Man Jiang, Daihyun Kim, Yoonhee Kim, Henry Kirkwood, Anna Klimovskaia, Juraj Knoska, Faisal H.M. Koua, Romain Letrun, Stella Lisova, Luis Maia, Victoria Mazalova, Domingo Meza, Thomas Michelat, Abbas Ourmazd, Guido Palmer, Marco Ramilli, Robin Schubert, Peter Schwander, Alessandro Silenzi, Jolanta Sztuk-Dambietz, Alexandra Tolstikova, Henry N. Chapman, Alexandra Ros, Anton Barty, Petra Fromme, Adrian P. Mancuso, Marius Schmidt

Molecular Sciences, School of (SMS)

Research output: Contribution to journal › Article › peer-review

100 Scopus citations

Abstract

The European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition rates. This X-ray source has been designed to enable the observation of ultrafast processes with near-atomic spatial resolution. Time-resolved crystallographic investigations on biological macromolecules belong to an important class of experiments that explore fundamental and functional structural displacements in these molecules. Due to the unusual MHz X-ray pulse structure at the EuXFEL, these experiments are challenging. Here, we demonstrate how a biological reaction can be followed on ultrafast timescales at the EuXFEL. We investigate the picosecond time range in the photocycle of photoactive yellow protein (PYP) with MHz X-ray pulse rates. We show that difference electron density maps of excellent quality can be obtained. The results connect the previously explored femtosecond PYP dynamics to timescales accessible at synchrotrons. This opens the door to a wide range of time-resolved studies at the EuXFEL.

Original language	English (US)
Pages (from-to)	73-78
Number of pages	6
Journal	Nature Methods
Volume	17
Issue number	1
DOIs	https://doi.org/10.1038/s41592-019-0628-z
State	Published - Jan 1 2020

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/s41592-019-0628-z

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Pandey, S., Bean, R., Sato, T., Poudyal, I., Bielecki, J., Cruz Villarreal, J., Yefanov, O., Mariani, V., White, T. A., Kupitz, C., Hunter, M., Abdellatif, M. H., Bajt, S., Bondar, V., Echelmeier, A., Doppler, D., Emons, M., Frank, M., Fromme, R., ... Schmidt, M. (2020). Time-resolved serial femtosecond crystallography at the European XFEL. Nature Methods, 17(1), 73-78. https://doi.org/10.1038/s41592-019-0628-z

Pandey, S, Bean, R, Sato, T, Poudyal, I, Bielecki, J, Cruz Villarreal, J, Yefanov, O, Mariani, V, White, TA, Kupitz, C, Hunter, M, Abdellatif, MH, Bajt, S, Bondar, V, Echelmeier, A, Doppler, D, Emons, M, Frank, M, Fromme, R, Gevorkov, Y, Giovanetti, G, Jiang, M, Kim, D, Kim, Y, Kirkwood, H, Klimovskaia, A, Knoska, J, Koua, FHM, Letrun, R, Lisova, S, Maia, L, Mazalova, V, Meza, D, Michelat, T, Ourmazd, A, Palmer, G, Ramilli, M, Schubert, R, Schwander, P, Silenzi, A, Sztuk-Dambietz, J, Tolstikova, A, Chapman, HN, Ros, A, Barty, A, Fromme, P, Mancuso, AP & Schmidt, M 2020, 'Time-resolved serial femtosecond crystallography at the European XFEL', Nature Methods, vol. 17, no. 1, pp. 73-78. https://doi.org/10.1038/s41592-019-0628-z

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title = "Time-resolved serial femtosecond crystallography at the European XFEL",

abstract = "The European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition rates. This X-ray source has been designed to enable the observation of ultrafast processes with near-atomic spatial resolution. Time-resolved crystallographic investigations on biological macromolecules belong to an important class of experiments that explore fundamental and functional structural displacements in these molecules. Due to the unusual MHz X-ray pulse structure at the EuXFEL, these experiments are challenging. Here, we demonstrate how a biological reaction can be followed on ultrafast timescales at the EuXFEL. We investigate the picosecond time range in the photocycle of photoactive yellow protein (PYP) with MHz X-ray pulse rates. We show that difference electron density maps of excellent quality can be obtained. The results connect the previously explored femtosecond PYP dynamics to timescales accessible at synchrotrons. This opens the door to a wide range of time-resolved studies at the EuXFEL.",

author = "Suraj Pandey and Richard Bean and Tokushi Sato and Ishwor Poudyal and Johan Bielecki and {Cruz Villarreal}, Jorvani and Oleksandr Yefanov and Valerio Mariani and White, {Thomas A.} and Christopher Kupitz and Mark Hunter and Abdellatif, {Mohamed H.} and Sa{\v s}a Bajt and Valerii Bondar and Austin Echelmeier and Diandra Doppler and Moritz Emons and Matthias Frank and Raimund Fromme and Yaroslav Gevorkov and Gabriele Giovanetti and Man Jiang and Daihyun Kim and Yoonhee Kim and Henry Kirkwood and Anna Klimovskaia and Juraj Knoska and Koua, {Faisal H.M.} and Romain Letrun and Stella Lisova and Luis Maia and Victoria Mazalova and Domingo Meza and Thomas Michelat and Abbas Ourmazd and Guido Palmer and Marco Ramilli and Robin Schubert and Peter Schwander and Alessandro Silenzi and Jolanta Sztuk-Dambietz and Alexandra Tolstikova and Chapman, {Henry N.} and Alexandra Ros and Anton Barty and Petra Fromme and Mancuso, {Adrian P.} and Marius Schmidt",

note = "Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.",

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T1 - Time-resolved serial femtosecond crystallography at the European XFEL

AU - Pandey, Suraj

AU - Bean, Richard

AU - Sato, Tokushi

AU - Poudyal, Ishwor

AU - Bielecki, Johan

AU - Cruz Villarreal, Jorvani

AU - Yefanov, Oleksandr

AU - Mariani, Valerio

AU - White, Thomas A.

AU - Kupitz, Christopher

AU - Hunter, Mark

AU - Abdellatif, Mohamed H.

AU - Bajt, Saša

AU - Bondar, Valerii

AU - Echelmeier, Austin

AU - Doppler, Diandra

AU - Emons, Moritz

AU - Frank, Matthias

AU - Fromme, Raimund

AU - Gevorkov, Yaroslav

AU - Giovanetti, Gabriele

AU - Jiang, Man

AU - Kim, Daihyun

AU - Kim, Yoonhee

AU - Kirkwood, Henry

AU - Klimovskaia, Anna

AU - Knoska, Juraj

AU - Koua, Faisal H.M.

AU - Letrun, Romain

AU - Lisova, Stella

AU - Maia, Luis

AU - Mazalova, Victoria

AU - Meza, Domingo

AU - Michelat, Thomas

AU - Ourmazd, Abbas

AU - Palmer, Guido

AU - Ramilli, Marco

AU - Schubert, Robin

AU - Schwander, Peter

AU - Silenzi, Alessandro

AU - Sztuk-Dambietz, Jolanta

AU - Tolstikova, Alexandra

AU - Chapman, Henry N.

AU - Ros, Alexandra

AU - Barty, Anton

AU - Fromme, Petra

AU - Mancuso, Adrian P.

AU - Schmidt, Marius

PY - 2020/1/1

Y1 - 2020/1/1

N2 - The European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition rates. This X-ray source has been designed to enable the observation of ultrafast processes with near-atomic spatial resolution. Time-resolved crystallographic investigations on biological macromolecules belong to an important class of experiments that explore fundamental and functional structural displacements in these molecules. Due to the unusual MHz X-ray pulse structure at the EuXFEL, these experiments are challenging. Here, we demonstrate how a biological reaction can be followed on ultrafast timescales at the EuXFEL. We investigate the picosecond time range in the photocycle of photoactive yellow protein (PYP) with MHz X-ray pulse rates. We show that difference electron density maps of excellent quality can be obtained. The results connect the previously explored femtosecond PYP dynamics to timescales accessible at synchrotrons. This opens the door to a wide range of time-resolved studies at the EuXFEL.

AB - The European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition rates. This X-ray source has been designed to enable the observation of ultrafast processes with near-atomic spatial resolution. Time-resolved crystallographic investigations on biological macromolecules belong to an important class of experiments that explore fundamental and functional structural displacements in these molecules. Due to the unusual MHz X-ray pulse structure at the EuXFEL, these experiments are challenging. Here, we demonstrate how a biological reaction can be followed on ultrafast timescales at the EuXFEL. We investigate the picosecond time range in the photocycle of photoactive yellow protein (PYP) with MHz X-ray pulse rates. We show that difference electron density maps of excellent quality can be obtained. The results connect the previously explored femtosecond PYP dynamics to timescales accessible at synchrotrons. This opens the door to a wide range of time-resolved studies at the EuXFEL.

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JF - Nature Methods

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Time-resolved serial femtosecond crystallography at the European XFEL

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Time-resolved serial femtosecond crystallography at the European XFEL

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Photoactive Yellow Protein PYP 80ps

Photoactive Yellow Protein PYP 10ps

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