Time-resolved protein nanocrystallography using an X-ray free-electron laser

Andrew Aquila, Mark S. Hunter, R. Bruce Doak, Richard Kirian, Petra Fromme, Thomas A. White, Jakob Andreasson, David Arnlund, Saša Bajt, Thomas R M Barends, Miriam Barthelmess, Michael J. Bogan, Christoph Bostedt, Hervé Bottin, John D. Bozek, Carl Caleman, Nicola Coppola, Jan Davidsson, Daniel P. Deponte, Veit Elser & 59 others Sascha W. Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Matthias Frank, Raimund Fromme, Heinz Graafsma, Ingo Grotjohann, Lars Gumprecht, Janos Hajdu, Christina Y. Hampton, Andreas Hartmann, Robert Hartmann, Stefan Hau-Riege, Günter Hauser, Helmut Hirsemann, Peter Holl, James M. Holton, André Hömke, Linda Johansson, Nils Kimmel, Stephan Kassemeyer, Faton Krasniqi, Kai Uwe Kühnel, Mengning Liang, Lukas Lomb, Erik Malmerberg, Stefano Marchesini, Andrew V. Martin, Filipe R N C Maia, Marc Messerschmidt, Karol Nass, Christian Reich, Richard Neutze, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Carlo Schmidt, Kevin Schmidt, Joachim Schulz, M. Marvin Seibert, Robert L. Shoeman, Raymond Sierra, Heike Soltau, Dmitri Starodub, Francesco Stellato, Stephan Stern, Lothar Strüder, Nicusor Timneanu, Joachim Ullrich, Xiaoyu Wang, Garth J. Williams, Georg Weidenspointner, Uwe Weierstall, Cornelia Wunderer, Anton Barty, John Spence, Henry N. Chapman

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

We demonstrate the use of an X-ray free electron laser synchronized with an optical pump laser to obtain X-ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. Light-induced changes of Photosystem I-Ferredoxin co-crystals were observed at time delays of 5 to 10 μs after excitation. The result correlates with the microsecond kinetics of electron transfer from Photosystem I to ferredoxin. The undocking process that follows the electron transfer leads to large rearrangements in the crystals that will terminally lead to the disintegration of the crystals. We describe the experimental setup and obtain the first timeresolved femtosecond serial X-ray crystallography results from an irreversible photo-chemical reaction at the Linac Coherent Light Source. This technique opens the door to time-resolved structural studies of reaction dynamics in biological systems.

Original languageEnglish (US)
Pages (from-to)2706-2716
Number of pages11
JournalOptics Express
Volume20
Issue number3
DOIs
StatePublished - Jan 30 2012

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free electron lasers
proteins
electron transfer
crystals
x rays
coherent light
disintegration
photochemical reactions
crystallography
nanocrystals
light sources
time lag
pumps
membranes
kinetics
liquids
diffraction
excitation
lasers

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Aquila, A., Hunter, M. S., Doak, R. B., Kirian, R., Fromme, P., White, T. A., ... Chapman, H. N. (2012). Time-resolved protein nanocrystallography using an X-ray free-electron laser. Optics Express, 20(3), 2706-2716. https://doi.org/10.1364/OE.20.002706

Time-resolved protein nanocrystallography using an X-ray free-electron laser. / Aquila, Andrew; Hunter, Mark S.; Doak, R. Bruce; Kirian, Richard; Fromme, Petra; White, Thomas A.; Andreasson, Jakob; Arnlund, David; Bajt, Saša; Barends, Thomas R M; Barthelmess, Miriam; Bogan, Michael J.; Bostedt, Christoph; Bottin, Hervé; Bozek, John D.; Caleman, Carl; Coppola, Nicola; Davidsson, Jan; Deponte, Daniel P.; Elser, Veit; Epp, Sascha W.; Erk, Benjamin; Fleckenstein, Holger; Foucar, Lutz; Frank, Matthias; Fromme, Raimund; Graafsma, Heinz; Grotjohann, Ingo; Gumprecht, Lars; Hajdu, Janos; Hampton, Christina Y.; Hartmann, Andreas; Hartmann, Robert; Hau-Riege, Stefan; Hauser, Günter; Hirsemann, Helmut; Holl, Peter; Holton, James M.; Hömke, André; Johansson, Linda; Kimmel, Nils; Kassemeyer, Stephan; Krasniqi, Faton; Kühnel, Kai Uwe; Liang, Mengning; Lomb, Lukas; Malmerberg, Erik; Marchesini, Stefano; Martin, Andrew V.; Maia, Filipe R N C; Messerschmidt, Marc; Nass, Karol; Reich, Christian; Neutze, Richard; Rolles, Daniel; Rudek, Benedikt; Rudenko, Artem; Schlichting, Ilme; Schmidt, Carlo; Schmidt, Kevin; Schulz, Joachim; Marvin Seibert, M.; Shoeman, Robert L.; Sierra, Raymond; Soltau, Heike; Starodub, Dmitri; Stellato, Francesco; Stern, Stephan; Strüder, Lothar; Timneanu, Nicusor; Ullrich, Joachim; Wang, Xiaoyu; Williams, Garth J.; Weidenspointner, Georg; Weierstall, Uwe; Wunderer, Cornelia; Barty, Anton; Spence, John; Chapman, Henry N.

In: Optics Express, Vol. 20, No. 3, 30.01.2012, p. 2706-2716.

Research output: Contribution to journalArticle

Aquila, A, Hunter, MS, Doak, RB, Kirian, R, Fromme, P, White, TA, Andreasson, J, Arnlund, D, Bajt, S, Barends, TRM, Barthelmess, M, Bogan, MJ, Bostedt, C, Bottin, H, Bozek, JD, Caleman, C, Coppola, N, Davidsson, J, Deponte, DP, Elser, V, Epp, SW, Erk, B, Fleckenstein, H, Foucar, L, Frank, M, Fromme, R, Graafsma, H, Grotjohann, I, Gumprecht, L, Hajdu, J, Hampton, CY, Hartmann, A, Hartmann, R, Hau-Riege, S, Hauser, G, Hirsemann, H, Holl, P, Holton, JM, Hömke, A, Johansson, L, Kimmel, N, Kassemeyer, S, Krasniqi, F, Kühnel, KU, Liang, M, Lomb, L, Malmerberg, E, Marchesini, S, Martin, AV, Maia, FRNC, Messerschmidt, M, Nass, K, Reich, C, Neutze, R, Rolles, D, Rudek, B, Rudenko, A, Schlichting, I, Schmidt, C, Schmidt, K, Schulz, J, Marvin Seibert, M, Shoeman, RL, Sierra, R, Soltau, H, Starodub, D, Stellato, F, Stern, S, Strüder, L, Timneanu, N, Ullrich, J, Wang, X, Williams, GJ, Weidenspointner, G, Weierstall, U, Wunderer, C, Barty, A, Spence, J & Chapman, HN 2012, 'Time-resolved protein nanocrystallography using an X-ray free-electron laser', Optics Express, vol. 20, no. 3, pp. 2706-2716. https://doi.org/10.1364/OE.20.002706
Aquila, Andrew ; Hunter, Mark S. ; Doak, R. Bruce ; Kirian, Richard ; Fromme, Petra ; White, Thomas A. ; Andreasson, Jakob ; Arnlund, David ; Bajt, Saša ; Barends, Thomas R M ; Barthelmess, Miriam ; Bogan, Michael J. ; Bostedt, Christoph ; Bottin, Hervé ; Bozek, John D. ; Caleman, Carl ; Coppola, Nicola ; Davidsson, Jan ; Deponte, Daniel P. ; Elser, Veit ; Epp, Sascha W. ; Erk, Benjamin ; Fleckenstein, Holger ; Foucar, Lutz ; Frank, Matthias ; Fromme, Raimund ; Graafsma, Heinz ; Grotjohann, Ingo ; Gumprecht, Lars ; Hajdu, Janos ; Hampton, Christina Y. ; Hartmann, Andreas ; Hartmann, Robert ; Hau-Riege, Stefan ; Hauser, Günter ; Hirsemann, Helmut ; Holl, Peter ; Holton, James M. ; Hömke, André ; Johansson, Linda ; Kimmel, Nils ; Kassemeyer, Stephan ; Krasniqi, Faton ; Kühnel, Kai Uwe ; Liang, Mengning ; Lomb, Lukas ; Malmerberg, Erik ; Marchesini, Stefano ; Martin, Andrew V. ; Maia, Filipe R N C ; Messerschmidt, Marc ; Nass, Karol ; Reich, Christian ; Neutze, Richard ; Rolles, Daniel ; Rudek, Benedikt ; Rudenko, Artem ; Schlichting, Ilme ; Schmidt, Carlo ; Schmidt, Kevin ; Schulz, Joachim ; Marvin Seibert, M. ; Shoeman, Robert L. ; Sierra, Raymond ; Soltau, Heike ; Starodub, Dmitri ; Stellato, Francesco ; Stern, Stephan ; Strüder, Lothar ; Timneanu, Nicusor ; Ullrich, Joachim ; Wang, Xiaoyu ; Williams, Garth J. ; Weidenspointner, Georg ; Weierstall, Uwe ; Wunderer, Cornelia ; Barty, Anton ; Spence, John ; Chapman, Henry N. / Time-resolved protein nanocrystallography using an X-ray free-electron laser. In: Optics Express. 2012 ; Vol. 20, No. 3. pp. 2706-2716.
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AU - Arnlund, David

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AU - Bostedt, Christoph

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AU - Deponte, Daniel P.

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AU - Epp, Sascha W.

AU - Erk, Benjamin

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AU - Foucar, Lutz

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AU - Fromme, Raimund

AU - Graafsma, Heinz

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AU - Wang, Xiaoyu

AU - Williams, Garth J.

AU - Weidenspointner, Georg

AU - Weierstall, Uwe

AU - Wunderer, Cornelia

AU - Barty, Anton

AU - Spence, John

AU - Chapman, Henry N.

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