Time-Resolved Digital Immunoassay for Rapid and Sensitive Quantitation of Procalcitonin with Plasmonic Imaging

Wenwen Jing; Yan Wang; Yunze Yang; Yi Wang; Guangzhong Ma; Shaopeng Wang; Nongjian Tao

doi:10.1021/acsnano.9b02771

Time-Resolved Digital Immunoassay for Rapid and Sensitive Quantitation of Procalcitonin with Plasmonic Imaging

Wenwen Jing, Yan Wang, Yunze Yang, Yi Wang, Guangzhong Ma, Shaopeng Wang, Nongjian Tao

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

Timely diagnosis of acute diseases improves treatment outcomes and saves lives, but it requires fast and precision quantification of biomarkers. Here, we report a time-resolved digital immunoassay based on plasmonic imaging of binding of single nanoparticles to biomarkers captured on a sensor surface. The real-time and high contrast of plasmonic imaging lead to fast and precise counting of the individual biomarkers over a wide dynamic range. We demonstrated the detection principle, evaluated the performance of the method using procalcitonin (PCT) as an example, and achieved a limit of detection of ∼2.8 pg/mL, dynamic range of 4.2-12500 pg/mL, for a total detection time of ∼25 min.

Original language	English (US)
Pages (from-to)	8609-8617
Number of pages	9
Journal	ACS nano
Volume	13
Issue number	8
DOIs	https://doi.org/10.1021/acsnano.9b02771
State	Published - Aug 27 2019

Keywords

plasmonic imaging
procalcitonin
rapid and sensitive quantification
single nanoparticles analysis
time-resolved digital immunoassay

ASJC Scopus subject areas

General Materials Science
General Engineering
General Physics and Astronomy

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10.1021/acsnano.9b02771

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title = "Time-Resolved Digital Immunoassay for Rapid and Sensitive Quantitation of Procalcitonin with Plasmonic Imaging",

abstract = "Timely diagnosis of acute diseases improves treatment outcomes and saves lives, but it requires fast and precision quantification of biomarkers. Here, we report a time-resolved digital immunoassay based on plasmonic imaging of binding of single nanoparticles to biomarkers captured on a sensor surface. The real-time and high contrast of plasmonic imaging lead to fast and precise counting of the individual biomarkers over a wide dynamic range. We demonstrated the detection principle, evaluated the performance of the method using procalcitonin (PCT) as an example, and achieved a limit of detection of ∼2.8 pg/mL, dynamic range of 4.2-12500 pg/mL, for a total detection time of ∼25 min.",

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author = "Wenwen Jing and Yan Wang and Yunze Yang and Yi Wang and Guangzhong Ma and Shaopeng Wang and Nongjian Tao",

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AU - Jing, Wenwen

AU - Wang, Yan

AU - Yang, Yunze

AU - Wang, Yi

AU - Ma, Guangzhong

AU - Wang, Shaopeng

AU - Tao, Nongjian

PY - 2019/8/27

Y1 - 2019/8/27

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AB - Timely diagnosis of acute diseases improves treatment outcomes and saves lives, but it requires fast and precision quantification of biomarkers. Here, we report a time-resolved digital immunoassay based on plasmonic imaging of binding of single nanoparticles to biomarkers captured on a sensor surface. The real-time and high contrast of plasmonic imaging lead to fast and precise counting of the individual biomarkers over a wide dynamic range. We demonstrated the detection principle, evaluated the performance of the method using procalcitonin (PCT) as an example, and achieved a limit of detection of ∼2.8 pg/mL, dynamic range of 4.2-12500 pg/mL, for a total detection time of ∼25 min.

KW - plasmonic imaging

KW - procalcitonin

KW - rapid and sensitive quantification

KW - single nanoparticles analysis

KW - time-resolved digital immunoassay

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Time-Resolved Digital Immunoassay for Rapid and Sensitive Quantitation of Procalcitonin with Plasmonic Imaging

Abstract

Keywords

ASJC Scopus subject areas

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