Time-Resolved Digital Immunoassay for Rapid and Sensitive Quantitation of Procalcitonin with Plasmonic Imaging

Wenwen Jing, Yan Wang, Yunze Yang, Yi Wang, Guangzhong Ma, Shaopeng Wang, Nongjian Tao

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Timely diagnosis of acute diseases improves treatment outcomes and saves lives, but it requires fast and precision quantification of biomarkers. Here, we report a time-resolved digital immunoassay based on plasmonic imaging of binding of single nanoparticles to biomarkers captured on a sensor surface. The real-time and high contrast of plasmonic imaging lead to fast and precise counting of the individual biomarkers over a wide dynamic range. We demonstrated the detection principle, evaluated the performance of the method using procalcitonin (PCT) as an example, and achieved a limit of detection of ∼2.8 pg/mL, dynamic range of 4.2-12500 pg/mL, for a total detection time of ∼25 min.

Original languageEnglish (US)
Pages (from-to)8609-8617
Number of pages9
JournalACS nano
Volume13
Issue number8
DOIs
StatePublished - Aug 27 2019

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Keywords

  • plasmonic imaging
  • procalcitonin
  • rapid and sensitive quantification
  • single nanoparticles analysis
  • time-resolved digital immunoassay

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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